Ethanol- induced chromosomal abnormalities in human fibroblast cells

authors:

avatar pooneh Aminigram , avatar Majid Rezaei Tavirani , avatar Mona Zamanian Azodi ORCID , * , avatar Reza Nasr , avatar Mohammad reza Akbari Eidgahi , avatar Bahador Bagheri ORCID

Koomesh: Vol.16, issue 2; 254-259
published online: September 28, 2015
article type: Research Article
received: April 01, 2014
accepted: October 02, 2014

how to cite: Aminigram P, Rezaei Tavirani M, Zamanian Azodi M, Nasr R, Akbari Eidgahi M R, et al. Ethanol- induced chromosomal abnormalities in human fibroblast cells. koomesh. 2015;16(2):e151306. 

Abstract

 Introduction: ethanol consumption may impose cytotoxic effects on the human body, by producing toxic metabolites through different pathways. Furthermore, some studies have also linked alcohol consumption to various types of diseases. Therefore, this in vitro study is aimed to assess the ethanol toxic effects on the human fibroblastic chromosome structure. Materials and Methods: G banding staining method was used to determine the karyotype of chromosomal abnormalities of cultured fibroblast cells. Karyogram of ethanol-treated cells after 48 hours of incubation with 54 and 108 mmol of ethanol were assessed against the untreated cultured cells. Results: the comparison between two karyogram models confirmed that lower ethanol concentration (54mmol) caused breakage in chromosome type C and number 1, while with higher concentration (108mmol) breakage was observed in chromosome 9, chromatids as well as endoreplication in some other cells. Conclusion: this study indicates that specific concentrations of ethanol can cause vast alterations in chromosomal structure. Therefore, in general, more care is suggested in consuming this substance. In addition, to confirm the cytotoxic alterations in chromosomal structure in relation to alcohol consumption, using other cell lines and /or in vivo studies, more investigations are warrant