A kinetic study on changes of mouse peripheral blood lymphocyte subsets after administration of T-2 toxin

How to Cite:Jafar SalimianNajmeh PoursasanFarid Aziz jalilianParviz KokhaieSona DalilanA kinetic study on changes of mouse peripheral blood lymphocyte subsets after administration of T-2 toxin.koomesh.16(2):e151307.

Abstract

Introduction: T-2 mycotoxin, makes contamination in food and feed and causes poisoning in human and livestock. T-2 toxin induces serious effect on immune system cells. In this study, kinetic of blood peripheral lymphocyte subsets after T-2 toxin exposure was investigated. Materials and Methods: In this experimental study, sublethal dose of T-2 toxin was determined and then injected into male mice. 12, 24, 48 and 72 h after toxin injection, a boold sample was taken from experimental anaimals. Afterward, kinetics of lymphocyte subpopulations such as CD19+, CD3+, CD4+ and CD8+ cells were analyzed using flow cytometry. Results: Sublethal dose of T-2 toxin was determined as 2 mg/kg. After sublethal dose injection CD19+ B cell significantly was decreased at 12 h and was returned to normal range at 3th day post-injection. CD3+ and CD4+ T populations increased at 24 h and were returned to normal range at 48 h, but inversely CD8+ T cells was decreased at 12 h and returned to to normal limit at 48 h. Conclusion: Alteration on blood lymphocytes due to T-2 toxin was occurred in 12 to 24 h post-injection. CD19+ and CD8+ lymphocytes were sensitive cells to toxin effect. CD3+ and CD4+ populations were increased. This may be due to pro-inflammatory cytokine release.T-2 toxin has different effects on kinetic of peripheral blood lymphocyte populations.

Keywords

زیر جمعیت‌های لنفوسیتی

سم T-2

سینتیک

فلوسایتومتر

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