Effects of different levels of Aloe Vera gel on some of hematological, biochemical and immunological parameters in the chicken model

authors:

avatar Ali Mahdavi , avatar Morteza Saberi , avatar Gholam-Ali Jeloudar , avatar E. Shahroozian , *

Koomesh: Vol.19, issue 1; 135-143
published online: September 28, 2017
article type: Research Article
received: December 05, 2015
accepted: September 01, 2016

how to cite: Mahdavi A, Saberi M, Jeloudar G, Shahroozian E. Effects of different levels of Aloe Vera gel on some of hematological, biochemical and immunological parameters in the chicken model. koomesh. 2017;19(1):e151330. 

Abstract

Introduction: The present study was conducted to investigate the effects of subchronic administration of different levels of Aloe Vera gel on some biochemical and immunological factors in the chicken model. Material and Methods: Two hundred and forty-one-day old Ross 308 (male and female) broilers were used on a completely randomized design in 5 groups with 4 replicates, and each replicate was consisting of 12 broilers. The groups included one control group (basal diet) and four experimental groups with basal diet mixed with different levels of Aloe Vera gel (1%, 2% and 3%) plus virginiamycin antibiotic. Results: No significant difference between experimental groups was observed in serum albumin level and ALP activity on days 14, 28 and 42. On the day 14, the variation of albumin and total protein levels were significant. In the days 28 and 42, the level of blood glucose in the group receiving 3% Aloe Vera and in the group receiving 1% Aloe Vera on the day 28 was decreased significantly. On the day  28, the count of lymphocytes was raised significantly in the group receiving 3% Aloe Vera. Also in this day, the most count of heterophils was found in the control and virginiamycin groups. On the day 42, the significant rise of lymphocyte count was observed in all groups receiving Aloe Vera gel. On the day  28, the level of antibody titers against sheep red blood cell was raised significantly. Conclusion: Daily supplementation with 1% Aloe Vera gel markedly potentiates cellular and humeral immunity in chickens and can be used as a food additive in order to prevent infections

References

  • 1.

    Ueda HR, Chen W, Adachi A, Wakamatsu H, Hayashi S, Takasugi T, et al. A transcription factor response element for gene expression during circadian night. Nature 2002; 418: 534-539.

  • 2.

    Akhtar M, Hai A, Awais MM, Iqbal Z, Muhammad F, ul Haq A, Anwar MI. Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens. Vet Parasitol 2012; 186: 170-177.

  • 3.

    Mashreghi M, Mollaie S, Gholami Z, Tavallaie S. Investigation on the effects of alcoholic extracts of fruit and leaves of three khorasani medicinal plants on Escherichia coli 0157 using spectrophotometery. Koomesh 2007; 8: 145-154. (Persian).

  • 4.

    Jebelli Javan A. Combinational effects of Trachyspermum ammi and Zataria multiflora Boiss essential oils on some pathogenic food-borne bacteria. Koomesh 2016; 17: 374-383. (Persian).

  • 5.

    Tan BK, Vanitha J. Immunomodulatory and antimicrobial effects of some traditional Chinese medicinal herbs: a review. Curr Med Chem 2004; 11: 1423-1430.

  • 6.

    Djeraba A, Quere P. In vivo macrophage activation in chickens with Acemannan, a complex carbohydrate extracted from Aloe vera. Int J Immunopharmacol 2000; 22: 365-372.

  • 7.

    yala I, Prez BG, Domnech G, Castells M, Valds M. Use of the chicken as an experimental animal model in atherosclerosis. Avian and Poultry Biology Reviews 2005; 16: 151-9.

  • 8.

    Mahdavi A, Shivazad M, Alemi F, Zaghari M, Moravej H, Darabighane B. Digestible lysine requirement of broilers based on practical diet. Italian Journal of Animal Science 2012; 11: 68-76.

  • 9.

    Kuehn L, Price S, Honaker C, Siegel P. Antibody response of chickens to sheep red blood cells: Crosses among divergently selected lines and relaxed sublines. Poult Sci 2006; 85: 1338-1341.

  • 10.

    Taiwo V, Olukunle O, Ozor I, Oyejobi A. Consumption of aqueous extract of raw Aloe vera leaves: histopathological and biochemical studies in rat and tilapia. African J Biomed Res 2006; 8: 169-178.

  • 11.

    Ajabnoor MA. Effect of aloes on blood glucose levels in normal and alloxan diabetic mice. J Ethnopharmacol 1990; 28: 215-220.

  • 12.

    Bunyapraphatsara N, Jirakulchaiwong S, Thirawarapan S, Manonukul J. The efficacy of Aloe vera cream in the treatment of first, second and third degree burns in mice. Phytomedicine 1996; 2: 247-251.

  • 13.

    al-Awadi F, Fatania H, Shamte U. The effect of a plants mixture extract on liver gluconeogenesis in streptozotocin induced diabetic rats. Diabetes Res 1991; 18: 163-168.

  • 14.

    Kaufman T, Kalderon N, Ullmann Y, Berger J. Aloe vera gel hindered wound healing of experimental second-degree burns: a quantitative controlled study. J Burn Care Rehabil 1988; 9: 156-159.

  • 15.

    Rajasekaran S, Ravi K, Sivagnanam K, Subramanian S. Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clin Exp Pharmacol Physiol 2006; 33: 232-237.

  • 16.

    Mehala C, Moorthy M. Production performance of broilers fed with Aloe vera and Curcuma longa (Turmeric). Int J Poult Sci 2008; 7: 852-856.

  • 17.

    Ur Rehman S, Durrani F, Chand N, Khan RU, ur Rehman F. Comparative efficacy of different schedules of administration of medicinal plants infusion on hematology and serum biochemistry of broiler chicks.ROAVS 2011.

  • 18.

    Mathivanan R, Kalaiarasi K. Panchagavya and Andrographis paniculata as alternatives to antibiotic growth promoters on haematological, serum biochemical parameters and immune status of broilers. J Poult Sci 2007; 44: 198-204.

  • 19.

    Viveros A, Brenes A, Arija I, Centeno C. Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poult Sci 2002; 81: 1172-1183.

  • 20.

    Singh J, Koley K, Chandrakar K, Pagrut NS. Effects of Aloe vera on dressing percentage and haemato-biochemidal parameters of broiler chickens. Veterinary World 2013; 6: 803-806.

  • 21.

    Fallah R. Effect of adding aloe vera gel and garlic powder on performance and liver functions of broiler chickens. Global J Anim Sci Res 2015; 3: 491-496.

  • 22.

    Calabuig CP, Ferrer M, Muriel R, Tilgar V. Plasma alkaline phosphatase as a sensitive indicator of age and skeletal development in wild coscoroba swans. Wildlife Research 2010; 37: 504-511.

  • 23.

    Lee A, Chui PT, Aun CS, Gin T, Lau AS. Possible interaction between sevoflurane and Aloe vera. Ann Pharmacother 2004; 38: 1651-1654.

  • 24.

    Tizard I. Immunity in the fetus and newborn animal. Introduct Vet Immunol 1982; 165-177.

  • 25.

    Kemper KJ, Small R. Astragalus (Astragalus membranaceous). Longwood Herbal Task Force 1999; 3: 1-18.

  • 26.

    Valle-Paraso D, Grace M, Vidamo D, Pernell Jan S, Anunciado D, Rea Victoria P, et al. Effects of aloe vera (Aloe barbadensis) on the white blood cell count and antibody titer of broiler chickens vaccinated against Newcastle disease. Philippine J Vet Med 2014; 42.

  • 27.

    eynolds T. Aloes: the genus Aloe: CRC press; 2004.

  • 28.

    Mmereole F. Evaluation of the dietary inclusion of Aloe vera as an alternative to antibiotic growth promoter in broiler production. Pakistan J Nutr 2011; 10: 1-5##.