Antibiotic resistance patterns and plasmid profiles of 200 clinically ioslated Staphyloococcus aureus

How to Cite:Ghorban BehzadianMasome AnoriAntibiotic resistance patterns and plasmid profiles of 200 clinically ioslated Staphyloococcus aureus.koomesh.2(2):e151920.

Abstract

Introduction. Many investigations have, so far , carried out to determine antibiotic resistance pattern and plasmid profiles . The aim of this study was to assess coagulase positive Staphylococcus resistance to 12 selected antibiotics, using disc diffusion and to determine their possible origin, through plasmid deletion, using mitomycin C treatment. Material and Methods. Bacterial strains were isolated from clinical specimens taken from patients in Imam Khorneini (RAH) hospital and preserved at our laboratory. Being cultured in nutrient and blood agar, they were incubated for 24 hours at 37 °C. Gram staining, coagulase and DNAase monitol fermentation tests were done. After preparing inoculating suspention, disc diffusion (12 antibiotics) test was performed. Susceptible and resistant strains were also determined through their treatment with mitomycin C. Results. The results indicated that antibi otic resistance was the most against erthrornycine (89%) and mitomycin C significantly decreased the frequency of single, double and multiple resistant cases. Conclusion. These findings suggest that coagulase positive, Staphylococcus aureus is resistant, to different degree, to some selected antibiotics . Plasmids not only cause resisance to aantibiotic but also play obvious role in multiple resistance.

Keywords

Plasmid, Mitomycin C, Staphyloococcus aureus, Resistance, Antibiotic

استافیلوکوکوس اورئوس، پلاسمید، میتومایسین C، کوآگولاز

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