Evaluating two indirect hemagglutination methods in diagnosis of listeriosis in comparison with indirect immunoflourecens

Bizhan SedighiMoghadam

Koomesh

Journal of Semnan University of Medical Sciences

Current Issue Archive In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Publication Malpractice Statement Support Contact Us Reviewer and AE Registration Form

APC

Authors Guide Submit Manuscript

Evaluating two indirect hemagglutination methods in diagnosis of listeriosis in comparison with indirect immunoflourecens

Author(s):

Bizhan SedighiMoghadam^,*

Koomesh:Vol. 3, issue 3; 123-129

Published online:Aug 15, 2002

Article type:Research Article

How to Cite:Bizhan SedighiMoghadamEvaluating two indirect hemagglutination methods in diagnosis of listeriosis in comparison with indirect immunoflourecens.koomesh.3(3):e151952.

Abstract

Introduction: Ba ct e rimi a res u lte d fro m Lis t eri a mono cyt ogen e s lea ds to va rIOUS complications including abortion . While cult uring the specimen is a crocial me thod in diagn osing of liste ri osi s, it has low se ns it ivity (because o f the few number of bacteria in specimens) and takes long time. Serological methods are used routinely in many diagnostic laboratories because of improved time and sensitivity. Materials and methods: IHA (indirect hemagglutination) was performed using fract ion 1, obtai ne d from ge l chroma tograp hy of sonica te d liste r ia monocytoge ne s cr ude antige ns. IHA th rou gh pot e l's me th od was ca rried on. Se ns it ivity and specific ity of th ese methods compa re d with current IFA (immunotlourecens assay) in diagnos ing the Listeriosis. The titers of antibody against Listeri a mon ocytoge nes were de te rmine d in se rums of 207 women having at le ast o ne abortion. Among this group serums of 96 women by IHA with soluble antigens in fra ction I and 82 women by IHA with soluble antigen in potel's method were tes ted . Results: In IFA (sensitivity 70% and spec ificity 50%),83.5% had valuable titer s of antibody. Niether crude antige n n or t hr ee fr ac tio ns we re suitab le for IHA, bu t al182 serums (pote l's method) showing 1/4 to 1/256 tite rs (se nsit ivity 36% and spec ificity 96%) . Conclusion: IFA is the most cu rrent method b ut, because o f h igh sensit ivity a nd low specific ity a more confident meth od is required. This study shows antigenic fraction s of listeria monocytoge nes are no t suitab le for IHA, while cha ng ing the co nd itio n of cu ltu re in po tel's method leads to the applicable antigen for IHA. Lack of correlation betwee n IFA and potel's IHA indicate that the nature of antigens arc diffe rent in bot h me thods. Thus it is recommended to use IFA for screening and simultaneously potel's IHA (because of high specificity).

Keywords

Indirect immunotlourecens (IFA), Indirect hemagglutinati on (IHA), Liste riosis

ایمونوفلورسانس غیرمستقیم، هماگلوتیناسیون غیرمستقیم، لیستریوز

comments