Detection and cloning of desulfurization operon sox(dszAB) in rhodococcus FMF native bacterium

How to Cite:Sodabeh AkbarzadesherbafJamshid RahebDetection and cloning of desulfurization operon sox(dszAB) in rhodococcus FMF native bacterium.koomesh.5(3):e152022.

Abstract

Introduction: Native Rhodococcus FMF bacterium was selected among several isolated Iranian bacterium. Primary studies have shown that the bacterium use dibenzothiophene as a sulfur source. Since desulfurization is taken by a biochemical pathway, the present project was designed to detect, clone and sequence of desulfurization genes of the bacterium. Materials and Methods: Desulfurization operon was isolated from Rhodococcus erythropolis IGTS8 and was used as a probe for detection of desulfurization 4S pathway genes by southern blotting technique. Specific primers were designed and the dszA and B genes were amplified by polymerase chain reaction (PCR). PCR products were recovered from agarose gel and were cloned into the PTZ57R vector. Results: In this study, recombinant PTZAB57R vector was constructed by the insertion of dszA and B genes into the PTZ57R vector. The clones were confirmed by restriction digestion analysis. The PTZAB57R was extracted by large-scale method and was sequenced (MWG DNA Biotech). Conclusion: Comparison of sequences of dszA and B genes from native Rhodococcus FMF bacterium with Rhodococcus sp. IGTS8 bacterium were shown complete identity between them, which show that desulfurization pathway is conserved in this bacterium.

Keywords

Rhodococcus sp., Rhodococcus erythropolis IGTS8, Biodesulfurization, Dibenzothiophene

رودوکوکوس، رودوکوکوس اریتروپولیس IGTS8، گوگردزدایی بیولوﮊیک، دی‌بنزوتیوفن

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