Study of XmnI polymorphism in 5´ region of the apoAI gene in Iranian hyperlipidemic subjects

authors:

avatar Ahmadreza Bandegi , * , avatar Mohsen FirozRay , avatar AbdolVahab EhsaniZenoz , avatar MohammadReza AkbariEyedgahi , avatar AliAkbar Shabani , avatar Mojtaba Malek

Koomesh: Vol.6, issue 1; 63-70
published online: November 15, 2004
article type: Research Article

how to cite: Bandegi A, FirozRay M, EhsaniZenoz A, AkbariEyedgahi M, Shabani A, et al. Study of XmnI polymorphism in 5´ region of the apoAI gene in Iranian hyperlipidemic subjects. koomesh. 2004;6(1):e152035. 

Abstract

Introduction: Several studies have demonstrated the relationship between polymorphisms in the ApoAI – CIII – AIV gene cluster and hyperlipidemia. This study was conducted to elucidate the association between polymorphism of ApoAI/XmnI and Iranian hyperlipidemic subjects. Materials and Methods: Total genomic DNA was prepared from seventy-six Iranian patient with primary hyperlipidemia and seventy-five normolipidemic subjects. The subjects in the control group were age- and sex-matched to the patients. Fragment of 392 bp for 5´ region of the apoAI gene (C-2500T) was amplified by polymerase chain reaction (PCR). Results: In the hyperlipidemic group, the genotype frequency of X1X1, X1X2, X2X2 were 0.63, 0.24, 0.13, respectively. In the control group those were 0.81, 0.11 and 0.08, respectivley. There was a significant difference (p