Introduce a model for diffuse brain injury in rats

Introduction: Diffuse brain injury occurs in nearly half of all severe of clinical traumatic brain injuries and has been associated with high mortality. Brain edema is a mortal pathological condition, which is commonly observed in diffuse brain trauma. In the current study, a new model producing diffuse brain injury, without focal brain lesions, was developed in rats, for first time in Iran. Matrials and Methods: Diffuse brain trauma was produced in 45 male rats that they were divided into three groups control, sham, and trauma group. Rats in two later groups underwent general anesthesia using a mixture of halothane, oxygen and nitrous oxide. A stainless steel disc (1cm in diameter) was firmly fixed to central portion of skull vault using dental acrylic. In the trauma group, a weight of 450g was allowed to drop from the 2m height onto the disc when trauma device was ready. All animal were mechanically ventilated during the treatment. In all groups, pathological studies and measurement of brain edema were performed at 24 hours following injury. Brain edema was evaluated by water content of brain and extravasation of Evans blue (E.B) dye. Results: The results of present study indicated that this technique induces a diffuse brain injury in the rats, as it was shown by a siginificant increase of brain water content in the trauma group compared to the control and the sham groups (p

Keywords

Brain trauma, Water content, Evans blue content, Brain edema

ترومای مغزی، محتوای آب، محتوای رنگ آبی ایوانز، خیز مغزی

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 5 days ago
Scopus by Title: 0
Last Update: 5 days ago
Scopus by Title (Ref): 0
Last Update: 5 days ago
CrossRef: 0
Last Update: 2 days ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Bahram Bibak:[PubMed][Scholar]
Mohammad Khaksari:[PubMed][Scholar]
Mohammad Badvi:[PubMed][Scholar]

Journal of Semnan University of Medical Sciences

Outlines

Introduce a model for diffuse brain injury in rats

Abstract

Last Update: 5 days ago

Last Update: 5 days ago

Last Update: 5 days ago

Last Update: 2 days ago