Abstract
Introduction: Epithelial is an important tissue in our body. The human epithelium forms a barrier between the external and internal environment. Natural process of wound healing e.g. ischemia, necrotic tissues and infection is a concept that has been in practice for a long time. Large full thickness wounds don’t heal spontaneously and are life treating. The closure of large wounds, when there is limited availability of autologous split skin grafts, has been a significant challenge to burns surgeons over the last century. Material & Methods: Rat keratinocytes were isolated from pups 1 DIV and cultured according to the to the protocol already described by Dulgos et al. Keratinocytes Isolated and were cultured in flask 75cm for 7 days ,then different concentrations of PDGF or GH were added daily for 6 days . Finally MTT test were done for all of wells(Case and Control) . Results were analyzed by Excel & SPSS. Results: Results of current study showed that keratinocytes culture were successfully established. There were two kinds of cells after 7 days in vitro. Both of the factors could stimulate cells reproduction. Count of cells between concentrations of 10 – 200 ng of PDGF was significant (P
Keywords
cell culture, PDGF, GH, keratinocyte. کشت سلول، کراتینوسیت، PDGF، GH
Copyright
© 2008, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.