IL-23 and IL-27 gene expression in three breast cancer cell lines and metastatic and non-metastatic lymph nodes in breast cancer patients

authors:

avatar Alireza Eskandari , avatar Mansooreh JabariPor , avatar Ali Khodadadi ORCID , * , avatar Abbas Ghaderi

Koomesh: Vol.12, issue 4; 428-434
published online: May 25, 2011
article type: Research Article
received: November 06, 2010
accepted: February 06, 2011

how to cite: Eskandari A, JabariPor M, Khodadadi A, Ghaderi A. IL-23 and IL-27 gene expression in three breast cancer cell lines and metastatic and non-metastatic lymph nodes in breast cancer patients. koomesh. 2011;12(4):e152477. 

Abstract

  Introduction: There is a great need to identify prognostic and diagnostic cancer bio-markers which can be applied for vaccine and immunotherapy development. The aim of this study was to determine gene expression of IL-23 and IL-27 in two groups: lymph nodes of breast cancer patients and three cell lines.   Materials and Methods: IL-23 and IL-27 mRNA transcript were investigated in 10 lymph node samples (consisting of 5 metastasis and 5 non metastasis ones) and three cell lines (including of SKBR3, MDA-MB-468 and MCF7) by quantitative real time PCR (Q-PCR) procedure using designed primers, Master Mix reaction containing SYBER green and β actin housekeeping gene.   Results: Data showed no significant differences in IL-23 and IL-27 mRNA gene expressions among metastatic lymph node and non-metastatatic ones. Also, we did not find any significant differences in IL-23 and IL-27 mRNA expressions in cancer cell lines.   Conclusions: Our findings indicate that breast cancer microenvironment has no effect on inflammation via either IL-23 gene expression or Il-27.