Effect of hydro-alcoholic extract of Vaccinium arctostaphylos on insulin release from rat-isolated langerhans islets

authors:

avatar Reza ShafieNik , * , avatar Seyyed Mohammad Reza Parizad , avatar Neda Zokaei , avatar Ahmad Ghorbani

Koomesh: Vol.12, issue 4; 447-451
published online: May 25, 2011
article type: Research Article
received: June 25, 2010
accepted: January 10, 2011

how to cite: ShafieNik R, Parizad S M R, Zokaei N, Ghorbani A. Effect of hydro-alcoholic extract of Vaccinium arctostaphylos on insulin release from rat-isolated langerhans islets. koomesh. 2011;12(4):e152479. 

Abstract

  Introduction: Vaccinium arctostaphylos is one of the medicinal plants used in the treatment of diabetes. But the mechanisms responsible for its antidiabetic action have not been well defined. This study was undertaken to investigate the possible effect of V. arctostaphylos on insulin release from rat-isolated Langerhans islets.   Materials and Methods : The islets were incubated in the presence of 3 or 10 mM glucose with or without milrinone (0.1 and 0.01%) and hydro-alcoholic extract of V. arctostaphylos (0.1 and 0.1%) for 60 min. The concentration of insulin was measured in samples of incubation medium.   Results: The increase of glucose concentration in incubation medium from 3 to 10 mM resulted in a significant increase of insulin secretion. Milrinone at 0.1 mM significantly increased insulin release evoked by 10 mM glucose. However, none of the extract concentrations affected the insulin release.   Conclusion: Our data demonstrated that V. arctostaphylos had no insulinotropic property and its effects on diabetes mediated by other mechanisms which should be clarified in future studies.