Effects of different phases of estrous cycle on brain edema and neurological outcomes after severe traumatic brain injury in female rats

authors:

avatar ali siahposhtkhachaki , avatar Mohammad KhakSariHadad , * , avatar nader ShahrokhiSardo , avatar ghplamreza Sepehri

Koomesh: Vol.13, issue 1; 62-72
published online: March 25, 2011
article type: issue_documents_importer
received: December 06, 2010
accepted: May 10, 2011

how to cite: siahposhtkhachaki A, KhakSariHadad M, ShahrokhiSardo N, Sepehri G. Effects of different phases of estrous cycle on brain edema and neurological outcomes after severe traumatic brain injury in female rats. koomesh. 2011;13(1):e152492. 

Abstract

  Introduction: Sex is one of the main recovery parameters after traumatic brain injury (TBI). During estrus cycle phase, plasma estrogen and progesterone levels are very different. So in this study, we have assessed different effects of these phases in brain edema and neurological outcomes.   Materials and Methods: Adult female Albino-N-MARI rats were divided into 6 groups (14 per group) including metestrus, diestrus, proestrus, estrus, ovarictomized (OVX) rats, and sham group (without TBI and ovary). In all groups, brain water content for the measurement of brain edema, evans blue content for the measurement of brain vascular permeability, neurological scores, plasma estrogen and progesterone levels were assessed after severe TBI.   Results: Brain water content in diestrus, proestrus and estrus groups showed a significant decrease as compared with OVX group (P