Interaction of glutamatergic system inhibition and aerobic exercise on anxiety-like behaviors in rats

How to Cite:Adele JafariMohammad RostampourAzam AtaeiBehrooz Khakpour TaleghaniInteraction of glutamatergic system inhibition and aerobic exercise on anxiety-like behaviors in rats.24(4):e152762.

Abstract

Introduction: Exploratory behaviors in rodents are an indicator of anxiety behaviors. In the present study, the combined effect of aerobic exercise with different intensities and inhibition of the glutamate system on exploratory behavior in rats was investigated. Materials and Methods: In this experimental study, 80 male Wistar rats were randomly divided into 10 groups including control, diazepam (0.6 mg/kg), two doses of MK-801 (0.1 and 0.05 mg/kg), two groups of acute aerobic exercise (low and moderate intensity), two acute aerobic exercise groups (low and moderate intensity) + dose 0.1 MK-801, the two groups performing acute aerobic exercise (low and moderate intensity) + dose 0.05 MK-801. On the test day, immediately after exerciseو drugs were injected, and 30 minutes later, exploratory behaviors were assessed by an open field. Results: Low and moderate-intensity exercise with MK-801 at a dose of 0.05 mg/kg significantly increased the frequency of rearing and decreased the number of grooming, and frequency of defecation compared to control and MK-801 groups (P

Keywords

لیسیدن و خاراندن بدن (تیمار کردن)

ورزش

References

1.
Poleszak E, Serefko A, Szopa A, Woko S, Dudka J, Wrbel A, et al. NMDA receptor activation antagonizes the NMDA antagonist-induced antianxiety effect in the elevated plus-maze test in mice. Pharmacol Rep 2013; 65: 1124-1131.https://doi.org/10.1016/S1734-1140(13)71470-1.
2.
Bowie D. Ionotropic glutamate receptors & CNS disorders. CNS Neurol Disord Drug Targets 2008; 7: 129-143.https://doi.org/10.2174/187152708784083821PMid:18537642 PMCid:PMC2662616.
3.
Blot K, Bai J, Otani S. The effect of non-competitive NMDA receptor antagonist MK-801 on neuronal activity in rodent prefrontal cortex: an animal model for cognitive symptoms of schizophrenia. J Physiol Paris 2013; 107: 448-451.https://doi.org/10.1016/j.jphysparis.2013.04.003PMid:23603055.
4.
Calcaterra NE, Barrow JC. Classics in chemical neuroscience: diazepam (valium). ACS Chem Neurosci 2014; 5: 253-260.https://doi.org/10.1021/cn5000056PMid:24552479 PMCid:PMC3990949.
5.
Heidary A, Emami A, Eskandaripour S, Saiah A, Hamidi S, Shahbazi M. Effects of aerobic exercise on anxiety. Proc Soc Behav Sci 2011; 30: 2497-2498. (Persian).https://doi.org/10.1016/j.sbspro.2011.10.487.
6.
Binder E, Droste SK, Ohl F, Reul JM. Regular voluntary exercise reduces anxiety-related behaviour and impulsiveness in mice. Behav Brain Res 2004; 155: 197-206.https://doi.org/10.1016/j.bbr.2004.04.017PMid:15364478.
7.
Vollert C, Zagaar M, Hovatta I, Taneja M, Vu A, Dao A, et al. Exercise prevents sleep deprivation-associated anxiety-like behavior in rats: potential role of oxidative stress mechanisms. Behav Brain Res 2011; 224: 233-240.https://doi.org/10.1016/j.bbr.2011.05.010PMid:21621560.
8.
Vafaei AA, Hagaygh KF, Seraj Z, Miladi Gorji H, Rashidy Pour A. Effects of forced exercise on object location memory and anxiety behaviour in morphine dependent ovariectomized rats. Koomesh 2020; 22: 704-710. (Persian).https://doi.org/10.29252/koomesh.22.4.704.
9.
DeBoer LB, Powers MB, Utschig AC, Otto MW, Smits JA. Exploring exercise as an avenue for the treatment of anxiety disorders. Expert Rev Neurother 2012; 12: 1011-1022.https://doi.org/10.1586/ern.12.73PMid:23002943 PMCid:PMC3501262.
10.
Broman-Fulks JJ, Berman ME, Rabian BA, Webster MJ. Effects of aerobic exercise on anxiety sensitivity. Behav Res Ther 2004; 42: 125-136.https://doi.org/10.1016/S0005-7967(03)00103-7.
11.
Zavvari F, Karimzadeh F. A methodological review of development and assessment of behavioral models of depression in rats. Neurosci J Shefaye Khatam 2015; 3: 151-160. (Persian).https://doi.org/10.18869/acadpub.shefa.3.4.151.
12.
Choleris E, Thomas AW, Kavaliers M, Prato FS. A detailed ethological analysis of the mouse open field test: effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field. Neurosci Biobehav Rev 2001; 25: 235-260.https://doi.org/10.1016/S0149-7634(01)00011-2.
13.
Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol 2003; 463: 3-33.https://doi.org/10.1016/S0014-2999(03)01272-X.
14.
Kalueff AV, Tuohimaa P. The grooming analysis algorithm discriminates between different levels of anxiety in rats: potential utility for neurobehavioural stress research. J Neurosci Methods 2005; 143: 169-177.https://doi.org/10.1016/j.jneumeth.2004.10.001PMid:15814150.
15.
Rostampour M, Hadipour E, Oryan S, Soltani B, Saadat F. Anxiolytic-like effect of hydroalcoholic extract of ripe pistachio hulls in adult female Wistar rats and its possible mechanisms. Res Pharm Sci 2016; 11: 454.https://doi.org/10.4103/1735-5362.194870PMid:28003838 PMCid:PMC5168881.
16.
Poleszak E, Wlaz P, Wrbel A, Fidecka S, Nowak G. NMDA/glutamate mechanism of magnesium-induced anxiolytic-like behavior in mice. Pharmacol Rep 2008; 60: 655.
17.
Otsuka T, Nishii A, Amemiya S, Kubota N, Nishijima T, Kita I. Effects of acute treadmill running at different intensities on activities of serotonin and corticotropin-releasing factor neurons, and anxiety-and depressive-like behaviors in rats. Behav Brain Res 2016; 298: 44-51.https://doi.org/10.1016/j.bbr.2015.10.055PMid:26542811.
18.
Szakacs R, Janka Z, Kalman J. The "blue" side of glutamatergic neurotransmission: NMDA receptor antagonists as possible novel therapeutics for major depression. Neuropsychopharmacol Hung 2012; 14: 29-40.
19.
Ennaceur A, Michalikova S, van Rensburg R, Chazot PL. MK-801 increases the baseline level of anxiety in mice introduced to a spatial memory task without prior habituation. Neuropharmacology 2011; 61: 981-991.https://doi.org/10.1016/j.neuropharm.2011.06.027PMid:21762710.
20.
Zarrindast MR, Nasehi M, Pournaghshband M, Yekta BG. Dopaminergic system in CA1 modulates MK-801 induced anxiolytic-like responses. Pharmacol Biochem Behav 2012; 103: 102-110.https://doi.org/10.1016/j.pbb.2012.07.016PMid:22885281.
21.
Patki G, Li L, Allam F, Solanki N, Dao AT, Alkadhi K, Salim S. Moderate treadmill exercise rescues anxiety and depression-like behavior as well as memory impairment in a rat model of posttraumatic stress disorder. Physiol Behav 2014; 130: 47-53.https://doi.org/10.1016/j.physbeh.2014.03.016PMid:24657739 PMCid:PMC4318362.
22.
Rashidy-Pour A, Vafaei AA, Mokhtari-Zaer A, Miladi-Gorji H. Physical activity alleviates anxiety but not hippocampal BDNF deficits in morphine abstinent rats. Koomesh 2018; 20: 594-602. (Persian).
23.
Tanaka S, Young JW, Halberstadt AL, Masten VL, Geyer MA. Four factors underlying mouse behavior in an open field. Behav Brain Res 2012; 233: 55-61.https://doi.org/10.1016/j.bbr.2012.04.045PMid:22569582 PMCid:PMC3866095.
24.
Koltyn KF. Analgesia following exercise. Sports Med 2000; 29: 85-98.https://doi.org/10.2165/00007256-200029020-00002PMid:10701712.
25.
Yuen EY, Jiang Q, Chen P, Gu Z, Feng J, Yan Z. Serotonin 5-HT1A receptors regulate NMDA receptor channels through a microtubule-dependent mechanism. J Neurosci 2005; 25: 5488-5501.https://doi.org/10.1523/JNEUROSCI.1187-05.2005PMid:15944377 PMCid:PMC6724987##.
26.
Boyce-Rustay JM, Holmes A. Genetic inactivation of the NMDA receptor NR2A subunit has anxiolytic-and antidepressant-like effects in mice. Neuropsychopharmacology 2006; 31: 2405-2414.https://doi.org/10.1038/sj.npp.1301039PMid:16482087.

comments