Evaluation of the Prognostic Value and TRIP13 gene Expression in Gastric Cancer

authors:

avatar Fariba Manzoori , avatar Mehrdad Nasrollahzadehsabet ORCID , avatar Arezoo Farhadi , avatar Reza Heidari , avatar Mohammad Foad Heidari , avatar Javad Behroozi ORCID , *

Koomesh: Vol.24, issue 6; 703-710
published online: December 03, 2022
article type: Research Article
received: January 02, 2021
accepted: May 03, 2022

how to cite: Manzoori F, Nasrollahzadehsabet M , Farhadi A, Heidari R, Heidari M F, et al. Evaluation of the Prognostic Value and TRIP13 gene Expression in Gastric Cancer. koomesh. 2022;24(6):e152782. 

Abstract

Introduction: Gastric cancer is a major public health issue worldwide. The factors that initiate cancer are not well understood, however aberrant expression of genes is associated with this cancer. TRIP13 plays pivotal roles in meiotic recombination, DNA repair, and cell cycle progression. An increasing body of evidence suggests that TRIP13 may possess functions other than meiosis and mitosis, particularly in the regulation of tumorigenicity. This study aimed to compare the expression of the TRIP13 gene in cancerous and adjacent tissue of gastric cancer and evaluate its clinical significance in the prognosis and survival analysis of gastric cancer patients. Materials and Methods: A total of 50 tumor tissues and marginal control tissues were obtained from gastric cancer patients. First, the relative gene expression of TRIP13 was determined using quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) and quantified using the 2-ΔΔCT method. Then, bioinformatic approaches were used to evaluate TRIP13 expression in two different cohorts of patients. A data mining study was also performed to determine the prognostic role of TRIP13 expression in the overall survival of gastric cancer patients. Finally, the correlation between gene expression and copy number of TRIP13 was obtained using CCLE database data analysis Results: The expression of TRIP13 in gastric tumor samples was increased 5.2-fold relative to adjacent normal tissues. Conspicuously, this finding was repeated in bioinformatics analyses and TRIP13 gene expression showed a significant increase in both cohorts of patients. Analysis of TRIP13 gene expression in 37 gastric cancer cell lines showed that the expression of this gene was significantly correlated with the number of copies. We also found a negative correlation between the high expression of TRIP13 and overall survival in gastric cancer patients. Conclusion: It could be concluded that TRIP13 may act as a promising biomarker and a potential therapeutic target for gastric cancer diagnosis and treatment.

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