Anti-cancer effects of the combined treatment of trastuzumab and decoy oligodeoxynucleotides to target STAT3 transcription factor on SK-BR-3 breast cancer cell line

Behrooz Johari; Roghayeh Ghorbani; Sara Heidari; Somayyeh Rashidi; Hamid Madanchi

Anti-cancer effects of the combined treatment of trastuzumab and decoy oligodeoxynucleotides to target STAT3 transcription factor on SK-BR-3 breast cancer cell line

authors:

Behrooz Johari , Roghayeh Ghorbani ^{, *} , Sara Heidari , Somayyeh Rashidi , Hamid Madanchi

Koomesh: Vol.25, issue 1; 71-82

article type: Research Article

received: June 01, 2022

accepted: November 03, 2022

how to cite: Johari B, Ghorbani R, Heidari S, Rashidi S, Madanchi H. Anti-cancer effects of the combined treatment of trastuzumab and decoy oligodeoxynucleotides to target STAT3 transcription factor on SK-BR-3 breast cancer cell line. koomesh. 2023;25(1):e152804.

Abstract

Introduction: Breast cancer is the most common malignancy in the female population and is the leading cause of death. Surgery, chemotherapy, radiotherapy, and monoclonal antibody (trastuzumab) therapy are common and standard treatments for this cancer. However, there are significant limitations in the treatment of this disease by using regular methods. Given the role of transcription factors (TFs) in gene expression, differentiation therapy, and many cancer signaling pathways, in the present study, the effect of combined treatment of trastuzumab (Herceptin) and decoy oligodeoxynucleotides to target the STAT3 transcription factor on inhibition of HER2 positive SK-BR-3 cell line growth was investigated. Materials and Methods: At first, different concentrations of Trastuzumab were prepared from 0.1 to 100 μg/mL. In this way, the decoy and scramble oligodeoxynucleotides were designed and synthesized to target the STAT3 transcription factor. The transfection efficiency and combined effects of trastuzumab with decoy oligos on cell viability, apoptosis, and cell migration inhibition were evaluated. Results: The results showed that oligodeoxynucleotides labeled with Cy3 fluorophore (300 nM) enter the cells with high efficiency (81.7±6.9%). Combined treatment of cells by trastuzumab (10 μg/mL) along with decoy oligodeoxynucleotides (100 nM) led to a decrease in cell viability (58.86±2.71%), increased apoptosis (24.54±4.83%) and inhibited cell migration (45.95±4.21%). Conclusion: The results from this study showed the anticancer effects of combination therapy using trastuzumab with decoy oligodeoxynucleotides to target the STAT3 transcription factor in SK-BR-3 cells. Therefore, it seems that this strategy can be used as an adjunct to common treatments for breast cancer.

Keywords

Breast cancer HER2 receptor Oligodeoxynucleotide decoy STAT3 transcription factor Apoptosis Cell migration سرطان پستان گیرنده HER2 الیگودئوکسی نوکلئوتید تله فاکتور رونویسی STAT3 آپوپتوز مهاجرت سلولی

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Anti-cancer effects of the combined treatment of trastuzumab and decoy oligodeoxynucleotides to target STAT3 transcription factor on SK-BR-3 breast cancer cell line

Abstract

Keywords

References

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