Effect of carnosine on cerebral edema and oxidative stress biomarkers in a transient model of focal cerebral ischemia in rats

authors:

avatar Abedin Vakili ORCID , * , avatar Ahmad Reza Bandegi , avatar Saeed Baiati , avatar Roya Hassanzadeh , avatar Yasin Asadi

Koomesh: Vol.15, issue 4; 493-501
published online: August 28, 2014
article type: Research Article
received: July 01, 2013
accepted: December 01, 2013

how to cite: Vakili A, Bandegi A R, Baiati S, Hassanzadeh R, Asadi Y. Effect of carnosine on cerebral edema and oxidative stress biomarkers in a transient model of focal cerebral ischemia in rats. koomesh. 2014;15(4):e152885. 

Abstract

 Introduction: Carnosine is an endogenous dipeptide expressed in many tissues including brain and has a protective effect against ischemic brain damage. However, its effect on brain edema, which is one of the most important variables in determining the amount of secondary brain damage after stroke, is not clear. The present study was conducted to investigate the effect of L-Carnosine on brain edema and oxidative stressbiomarkers in an experimental model of focal cerebral ischemia. Materials and Methods: Under Laser Doppler flowmetry, cerebral ischemia was induced by transient occlusion of middle cerebral artery for 1 hour in rats. L-Carnosine at doses of 250 and 500 mg/kg.ip was injected immediately after induction of ischemia. Cerebral edema and enzymes activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) content were measured 24 hours after ischemia using a standard method and kit. Results: Administration of L-Carnosine at doses 250(%81.5±0.36) and 500 (%80.9±0.30) mg/kg significantly reduced brain edema by 25% and 40% in comparison with the control group (%82.53±0.16), respectively (P