Inhibitory effect of luteolin on MKN-45 tumorg gastric cells: The role of nucleoside-diphosphate kinases, cofilin and actin proteins

authors:

avatar Zahra Tahmasebi , avatar Maziar Akhavan , * , avatar Sepideh Torabi , avatar Naser Abbasi , avatar Elham Sarhadi , avatar NAsim Hatam nejadian

Koomesh: Vol.19, issue 3; 611-618
published online: July 08, 2017
article type: Research Article
received: July 02, 2016
accepted: April 08, 2017

how to cite: Tahmasebi Z, Akhavan M, Torabi S, Abbasi N, Sarhadi E, et al. Inhibitory effect of luteolin on MKN-45 tumorg gastric cells: The role of nucleoside-diphosphate kinases, cofilin and actin proteins. koomesh. 2017;19(3):e152907. 

Abstract

Introduction: Luteolin is an important plant extract. This shows anti-inflammatory, inhibition of cell growth, induction of apoptosis and inhibition of metastasis. Gastric cancer is one of the most common cancers in the world. Also metastasis is widespread in this kind of cancer and late diagnosis is a cause of patient;#39s death.  Cofilin, actin and nucleoside-diphosphate kinases (NDPK) are important in cell death and metastasis. In this study the effect of luteolin on MKN-45 gastric carcinoma cells was evaluated. Materials and Methods: At first the cells were cultured and then for 48 hours were cultured with the drug concentration. Then the cells where collected and their protein were extracted and analyzed using 2D electrophoresis and separated on the basis of their molecular mass and PI. After that these samples were used for determine the amino acid sequence by MOLDI-TOF. Results: The growth rate of MKN-45 inhibited under influence of luteolin, this means increasing Apoptosis. Based on the results presented here by identification of protein spots by MOLDI-TOF mass spectrometry the expression of three protein cofilin, actin and NDPK has been decreased by luteolin. Conclusion: It seems that the anti- tumoral effect of luteolin is expressed through three proteins cofilin, actin and NDPK in gastric cancer cells

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