Effects of IL-4 and RANKL synergism on survival of peripheral blood monocytes in patients with giant cell granuloma

authors:

avatar Leila Mohamed Khosroshahi , avatar leili Aghebati-Maleki , avatar Sona Rafieyan , avatar A. li Aghbali , avatar A. li Aghebati , avatar A. li Fotouhi , avatar leili Aghebati-Maleki , * , avatar Behzad Baradaran

Koomesh: Vol.21, issue 1; 140-144
published online: March 02, 2019
article type: Research Article
received: July 01, 2017
accepted: June 02, 2018

how to cite: Mohamed Khosroshahi L, Aghebati-Maleki L, Rafieyan S, Aghbali A L, Aghebati A L, et al. Effects of IL-4 and RANKL synergism on survival of peripheral blood monocytes in patients with giant cell granuloma. koomesh. 2019;21(1):e153049. 

Abstract

Introduction: Giant cell granuloma is a non-neoplastic lesion that is characterized by the fact that granulation tissue proliferation has many multiples of giant cells.Considering the role of IL-4 and RANKL in the pathogens of the disease in this study, we investigated the synergism effects of these two factors on the survival of monocytes isolated from peripheral blood in patients with giant cell granuloma compared with healthy subjects. Materials and Methods: After blood collection of patients with giant cell granuloma and healthy individuals, peripheral blood mononuclear cells were isolated by ficoll density gradient centrifugation and the monocytes were isolated using human Monocyte Isolation Kit II. Isolated monocytes were then cultured in the absence or presence of IL-4 and RANKL (10 and 20 ng/mL) for five days. After morphological examination by inverted microscope, following MTT assay was performed to determine proliferation. Results: The results showed a difference in the survival of monocytes in comparison between the patient group and the healthy group after the treatment period. On the other hand, in both patient and healthy groups, the number of treated cells was higher in comparison to untreated control groups, which in the groups treated with morphological evidence showed a change in favor of the formation of giant cells. Both IL-4 and RANKL factors increased the survival of monocytes in comparison with the control group. Conclusion: The results of this study showed the increased survival and the number of monocytes isolated from peripheral blood in patients with giant cell granuloma by IL-4 and RANKL factors, with the creation of morphology of giant cell. These results indicate that IL-4 and RANKL factors are involved in the onset of malignant disease

References

  • 1.

    Spraggs P, Roth J, Young-Ramsaran J, Goodwin WJ. Giant cell reparative granuloma of the maxilla.Ear Nose Throat J 1997; 76:445-446, 449.

  • 2.

    Tsichlaki A, George KS, Manisali M. An unusual presentation of a maxillary central giant cell granuloma.J Surg Case Rep 2012; 2012:7.

  • 3.

    Pogrel AM. The diagnosis and management of giant cell lesions of the jaws.Ann MaxillofacSurg2012; 2:102-106.

  • 4.

    Vignery A. Osteoclasts and giant cells: macrophage-macrophage fusion mechanism. Int J ExpPathol2000; 81:291-304.

  • 5.

    Khoramian S, Poureslami H, Mohammadi B. Central giant cell granuloma: (A Case Report). J RafsanjanUniv Med Sci 2012; 11:503-510. (Persian).

  • 6.

    Gordon S, Taylor PR. Monocyte and macrophage heterogeneity. Nat Rev Immunol2005;5:953-964.

  • 7.

    Pak F, Barati M, Shokrolahi M, Kokhaei P. Tumor immunology and tumor escape mechanisms from immune response. Koomesh 2014; 15:412-430. (Persian).

  • 8.

    Lorenzo J, Horowitz M, Choi Y. Osteoimmunology: interactions of the bone and immune system. Endocr Rev 2008; 29:403-440.

  • 9.

    Moreno JL, Mikhailenko I, Tondravi MM, Keegan AD. IL-4 promotes the formation of multinucleated giant cells from macrophage precursors by a STAT6-dependent, homotypic mechanism: contribution of E-cadherin. J LeukocBiol 2007; 82:1542-1553.

  • 10.

    Yu M, Qi X, Moreno JL, Farber DL, Keegan AD. NF-kappaB signaling participates in both RANKL- and IL-4-induced macrophage fusion: receptor cross-talk leads to alterations in NF-kappaB pathways. J Immunol 2011; 187:1797-1806.

  • 11.

    Mirmohammadkhani O, Motahari P, Ghanadan A, Chahardehi R, Mirmohammadkhani M. Activated leukocyte cell adhesion molecule expression in oral squamus cell carcinoma and its association with clinical and histopathologic parameters. Koomesh 2013; 14:350-356.

  • 12.

    Moreno JL, Kaczmarek M, Keegan AD, Tondravi M. IL-4 suppresses osteoclast development and mature osteoclast function by a STAT6-dependent mechanism: irreversible inhibition of the differentiation program activated by RANKL. Blood 2003; 102:1078-1086.

  • 13.

    Breuil V, Schmid-Antomarchi H, Schmid-Alliana A, Rezzonico R, Euller-Ziegler L, Rossi B. The receptor activator of nuclear factor (NF)-kappaB ligand (RANKL) is a new chemotactic factor for human monocytes. FASEB J 2003; 17:1751-1753.

  • 14.

    Hoffmann A, Baltimore D. Circuitry of nuclear factor kappaB signaling. Immunol Rev2006;210:171-186.

  • 15.

    Aghbali A, Rafieyan S, Mohamed-Khosroshahi L, Baradaran B, Shanehbandi D, Kouhsoltani M. IL-4 induces the formation of multinucleated giant cells and expression of beta5 integrin in central giant cell lesion. Med Oral Patol Oral Cir Bucal 2017; 22:e1-e6.

  • 16.

    Regezi JA. Comments on the pathogenesis and medical treatment of central giant cell granulomas.J Oral MaxillofacSurg 2004;62:116-118.

  • 17.

    Kruse-Lsler B, Diallo R, Gaertner C, Mischke KL, Joos U, Kleinheinz J. Central giant cell granuloma of the jaws: a clinical, radiologic, and histopathologic study of 26 cases. Oral Surg Oral Med Oral Pathol Oral RadiolEndod 2006; 101:346-354.

  • 18.

    Eckardt A, Pogrel MA, Kaban LB, Chew K, Mayall BH. Central giant cell granulomas of the jaws: nuclear DNA analysis using image cytometry. Int J Oral MaxillofacSurg 1989; 18:3-6.

  • 19.

    Whitaker SB, Waldron CA. Central giant cell lesions of the jaws: a clinical, radiologic, and histopathologic study. Oral Surg Oral Med Oral Pathol 1993; 75:199-208.

  • 20.

    Lim L, Gibbins JR. Immunohistochemical and ultrastructural evidence of a modified microvasculature in the giant cell granuloma of the jaws. Oral Surg Oral Med Oral Pathol Oral RadiolEndod 1995; 79:190-198.

  • 21.

    Huang L, Xu J, Wood DJ, Zheng MH. Gene expression of osteoprotegerin ligand, osteoprotegerin, and receptor activator of NF-B in giant cell tumor of bone: possible involvement in tumor cell-induced osteoclast-like cell formation. Am J Pathol 2000; 156:761-767. ##.