In vitro anti-bacterial effect of nano-polyamidoamine-G5 dendrimer

authors:

avatar Soudabeh Alizadeh Matboo , avatar Shahram Nazari , * , avatar Leila Mohammadi , avatar Susan Bagheri , avatar Fakhraddin Akbari Dourbash , avatar Ahmadreza Yari , avatar Seyyed Ahmad Mokhtari , avatar A. li Niapour , avatar Seyyed Mohsen Mohseni

Koomesh: Vol.21, issue 1; 171-180
published online: March 01, 2019
article type: Research Article
received: September 01, 2016
accepted: August 01, 2018

how to cite: Alizadeh Matboo S, Nazari S, Mohammadi L, Bagheri S, Akbari Dourbash F, et al. In vitro anti-bacterial effect of nano-polyamidoamine-G5 dendrimer. koomesh. 2019;21(1):e153054. 

Abstract

Introduction: Progress in nanotechnology in the past decayed has created various opportunities for evaluation of biological effects such as anti-bacterial effects of nanoparticles.  This study was aimed to examine synthesis and the antibacterial effect of Nano-Polyamidoamine-G5 (NPAMAM-G5) dendrimer on Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis. Materials and Methods:  NPAMAM-G5 dendrimers was synthesized by Tomalia’s divergent growth approach. The antibacterial effects of NPAMAM-G5 dendrimer were studied by disc diffusion and micro-dilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against gram-positive and gram-negative bacteria were determined according to Clinical and Laboratory Standards Institute (CLSI) guideline. Transmission electron microscopy (TEM) was used to analyze morphology and size of NPAMAM-G5. Results: Zone of inhibition in concentration 25μg/ml of NPAMAM-G5 dendrimers for Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis were 27, 13, 30 and 18 mm, respectively. There was a significant difference regarding the zone of inhibition between gram-negative and gram-positive bacteria (p

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