Analysis of epithelial mesenchymal transition markers in breast cancer cells in response to stromal cell-derived factor 1

authors:

avatar Naghmeh Ahmadiankia , *

Koomesh: Vol.21, issue 2; 306-311
published online: May 02, 2019
article type: Research Article
received: April 01, 2018
accepted: October 02, 2018

how to cite: Ahmadiankia N. Analysis of epithelial mesenchymal transition markers in breast cancer cells in response to stromal cell-derived factor 1. koomesh. 2019;21(2):e153072. 

Abstract

Introduction: Metastasis is the main cause of cancer death however, the underlying mechanisms of metastasis are largely unknown. The chemokine of stromal cell-derived factor 1 (SDF1) and the process of epithelial mesenchymal transition (EMT), both have been declared as important factors to promote cancer metastasis however, Conspicuously, the relation between them has not been recognized well yet. Due to the importance of investigating the factors involved in cancer metastasis to develop new therapeutic strategies, in this study the effect of SDF1 on the expression of transcription factors involved in EMT was analyzed in breast cancer cells. Materials and Methods: MDA-MB-361 cells were treated with 100 ng/ml SDF1 in different time points of 24, 48, 72 and 96 hours. Then the expression of EMT markers were analyzed by Real time PCR (Cyber green) and western blot methods. Results: Snail and Zeb1 genes had upregulation in response to SDF1 treatment (P

References

  • 1.

    Boyle P. Triple-negative breast cancer: epidemiological considerations and recommendations. Ann Oncol Off J Eur Soc Med Oncol 2012; 23: vi7-12.

  • 2.

    Seyfried TN, Huysentruyt LC. On the origin of cancer metastasis. Crit Rev Oncog 2013; 18: 43-73.

  • 3.

    Mishan MA, Ahmadiankia N, Bahrami AR. CXCR4 and CCR7: Two eligible targets in targeted cancer therapy. Cell Biol Int 2016; 40: 955-967.

  • 4.

    Ray P, Lewin SA, Mihalko LA, Lesher-Perez S-C, Takayama S, Luker KE, et al. Secreted CXCL12 (SDF-1) forms dimers under physiological conditions. Biochem J 2012; 442: 433-442.

  • 5.

    Papatheodorou H, Papanastasiou AD, Sirinian C, Scopa C, Kalofonos HP, Leotsinidis M, et al. Expression patterns of SDF1/CXCR4 in human invasive breast carcinoma and adjacent normal stroma: correlation with tumor clinicopathological parameters and patient survival. Pathol Res Pract 2014; 210: 662-667.

  • 6.

    Sun Y, Mao X, Fan C, Liu C, Guo A, Guan S, et al. CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis. Tumour Biol 2014; 35: 7765-7773.

  • 7.

    Li YP, Pang J, Gao S, Bai P-Y, Wang W, Kong P, et al. Role of CXCR4 and SDF1 as prognostic factors for survival and the association with clinicopathology in colorectal cancer: A systematic meta-analysis. Tumour Biol 2017; 39: 1010428317706206.

  • 8.

    Zheng N, Chen J, Liu W, Liu J, Li T, Chen H, et al. Mifepristone inhibits ovarian cancer metastasis by intervening in SDF-1/CXCR4 chemokine axis. Oncotarget 2017; 8: 59123-59135.

  • 9.

    Liepelt A, Tacke F. Stromal cell-derived factor-1 (SDF-1) as a target in liver diseases. Am J Physiol Gastrointest Liver Physiol 2016; 311: G203-209.

  • 10.

    Tseng D, Vasquez-Medrano DA, Brown JM. Targeting SDF-1/CXCR4 to inhibit tumour vasculature for treatment of glioblastomas. Br J Cancer 2011; 104: 1805-1809.

  • 11.

    Brabletz T, Kalluri R, Nieto MA, Weinberg RA. EMT in cancer. Nat Rev Cancer 2018; 18: 128-134.

  • 12.

    Heerboth S, Housman G, Leary M, Longacre M, Byler S, Lapinska K, et al. EMT and tumor metastasis. Clin Transl Med 2015; 4: 6.

  • 13.

    Comito G, Giannoni E, Segura CP, Barcellos-de-Souza P, Raspollini MR, Baroni G, et al. Cancer-associated fibroblasts and M2-polarized macrophages synergize during prostate carcinoma progression. Oncogene 2014; 33: 2423-2431.

  • 14.

    Mishan MA, Heirani-Tabasi A, Mokhberian N, Hassanzade M, Kalalian Moghaddam H, Bahrami AR, et al. Analysis of chemokine receptor gene epression in esophageal cancer cells compared with breast cancer with insights into metastasis. Iran J Public Health 2015; 44: 1353-1358.

  • 15.

    Ahmadiankia N, Moghaddam HK, Mishan MA, Bahrami AR, Naderi-Meshkin H, Bidkhori HR, et al. Berberine suppresses migration of MCF-7 breast cancer cells through down-regulation of chemokine receptors. Iran J Basic Med Sci 2016; 19: 125-131.

  • 16.

    Bagheri M, Fazli M, Saeednia S, Kor A, Ahmadiankia N. Pomegranate peel extract inhibits expression of beta-catenin, epithelial mesenchymal transition, and metastasis in triple negative breast cancer cells. Cell Mol Biol (Noisy-le-grand) 2018; 64: 86-91.

  • 17.

    Dillenburg-Pilla P, Patel V, Mikelis CM, Zarate-Blades CR, Doci CL, Amornphimoltham P, et al. SDF-1/CXCL12 induces directional cell migration and spontaneous metastasis via a CXCR4/Galphai/mTORC1 axis. FASEB J Off Publ Fed Am Soc Exp Biol 2015; 29: 1056-1068.

  • 18.

    Faber A, Goessler UR, Hoermann K, Schultz JD, Umbreit C, Stern-Straeter J. SDF-1-CXCR4 axis: cell trafficking in the cancer stem cell niche of head and neck squamous cell carcinoma. Oncol Rep 2013; 29: 2325-2331.

  • 19.

    Yi T, Zhai B, Yu Y, Kiyotsugu Y, Raschle T, Etzkorn M, et al. Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 2014; 111: E2182-2190.

  • 20.

    Song Z, Zhang X, Ye X, Feng C, Yang G, Lu Y, et al. High expression of stromal cell-derived factor 1 (SDF-1) and NF-kappaB predicts poor prognosis in cervical cancer. Med Sci Monit 2017; 23: 151-157.

  • 21.

    Liu H, Li Z, Deng M, Liu Q, Zhang T, Guo W, et al. Prognostic and clinicopathological value of CXCL12/SDF1 expression in breast cancer: A meta-analysis. Clin Chim Acta 2018; 484: 72-80.

  • 22.

    Wang L, Chen W, Gao L, Yang Q, Liu B, Wu Z, et al. High expression of CXCR4, CXCR7 and SDF-1 predicts poor survival in renal cell carcinoma. World J Surg Oncol 2012; 10: 212.

  • 23.

    Bouyssou JMC, Ghobrial IM, Roccaro AM. Targeting SDF-1 in multiple myeloma tumor microenvironment. Cancer Lett 2016; 380: 315-318.

  • 24.

    Wong SH, Fang CM, Chuah LH, Leong CO, Ngai SC. E-cadherin: Its dysregulation in carcinogenesis and clinical implications. Crit Rev Oncol Hematol 2018; 121: 11-22.

  • 25.

    Mezencev R, Matyunina L V, Jabbari N, McDonald JF. Snail-induced epithelial-to-mesenchymal transition of MCF-7 breast cancer cells: systems analysis of molecular changes and their effect on radiation and drug sensitivity. BMC Cancer 2016; 16: 236.

  • 26.

    Wang Y, Shi J, Chai K, Ying X, Zhou BP. The role of snail in EMT and tumorigenesis. Curr Cancer Drug Targets 2013; 13: 963-972.

  • 27.

    Suh Y, Yoon CH, Kim RK, Lim EJ, Oh YS, Hwang SG, et al. Claudin-1 induces epithelial-mesenchymal transition through activation of the c-Abl-ERK signaling pathway in human liver cells. Oncogene 2013; 32: 4873-4882.

  • 28.

    Onoue T, Uchida D, Begum NM, Tomizuka Y, Yoshida H, Sato M. Epithelial-mesenchymal transition induced by the stromal cell-derived factor-1/CXCR4 system in oral squamous cell carcinoma cells. Int J Oncol 2006; 29: 1133-1138##.