Effects of oral gavage treatment of Eupatilin on protection of skin flaps in rats

authors:

avatar Jalaledin Mirzay Razaz , avatar Amir ali Ebadi Fard Fzar , avatar Mahdyieh Naziri , avatar Majid Banimohammad , avatar Abbas Majdi Seghinsara , avatar Gholamali Javdan , *

Koomesh: Vol.21, issue 2; 318-323
published online: May 02, 2019
article type: Research Article
received: May 01, 2018
accepted: January 02, 2019

how to cite: Razaz J M, Ebadi Fard Fzar A A, Naziri M, Banimohammad M, Majdi Seghinsara A, et al. Effects of oral gavage treatment of Eupatilin on protection of skin flaps in rats. koomesh. 2019;21(2):e153074. 

Abstract

Introduction: Nowadays, skin flaps are used to repair wounds, skin lesions and in reconstructive surgeries. In this way, the main problem is loss of flap tissue and necrosis, due to processes that result from ischemia-reperfusion injury and reactive oxygen species. Eupatilin is an O-methylated flavone derived from Artemisia plants that demonstrates anti-inflammatory, anti-oxidative, and anti-tumor effects. In this study, we evaluated the effect of Eupatilin administration on the survival of skin flap and oxidative stress by measuring necrotic area of skin flap and oxidative stress markers, in rats. Materials and Methods: Forty rats were divided into four groups (10 rats per group), including Sham group, Flap group, Eupatilin+ Flap group (received Eupatilin before flap), and Flap + Eupatilin group (received Eupatilin after flap). Eupatilin (10 mg / kg) was administered as oral gavage to mice. One week after flap surgery in a 3 x 8 cm scale, flap necrosis, malondialdehyde (MDA) levels - using thiobarbituric acid method (TBA)- and superoxide dismutase (SOD) - using an oxidase enzymatic method- was measured. Results: Treatment with Eupatilin, before or after flap, reduced the necrosis area of flap (P

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