Cytotoxicity effect of gold nanoparticles against a rabbit model of retinoblastoma

authors:

avatar Somayeh Moradi , avatar Manijheh Mokhtari ORCID , * , avatar Fariba Ghassemi , avatar Shahab Sheibani , avatar Fahimeh Asadi Amoli

Koomesh: Vol.21, issue 4; 726-734
published online: December 01, 2019
article type: Research Article
received: January 01, 2019
accepted: May 01, 2019

how to cite: Moradi S, Mokhtari M, Ghassemi F, Sheibani S, Asadi Amoli F. Cytotoxicity effect of gold nanoparticles against a rabbit model of retinoblastoma. koomesh. 2019;21(4):e153138. 

Abstract

Introduction: Retinoblastoma is a malignant tumor in children. The goal of retinoblastoma treatment is to maintain vision and minimize side effects. In this study, the toxicity of the gold nanoparticle safety in vitro was investigated on an eye tumor of retinoblastoma under in vivo conditions. Materials and Methods: For the evaluation of the toxicity of gold nanoparticles using MTT test after 48 hours in a CO2 incubator at 37 ° C, Y79 cell line was administered under in vitro conditions at concentrations of 1.69, 1.75, 3.5, 7, 14 and 28 μg/ml of gold nanoparticles.  Safe dose in vitro was extracted. The toxicity of gold nanoparticles in the treatment of retinoblastoma tumor under in vivo conditions in a rabbit animal model using histochemistry analysis and hemoxin-eosin staining, as well as measuring the cross-sectional area of the tumor using ultrasonic sequential image analysis and extraction using software Image J was studied. Results: The results showed that with increasing the concentration of gold nanoparticles, the percentage of live cells in comparison with the control group had a significant difference (p 0.05) in the concentration of 1.69 μg/ml. The results indicated that the injection of gold nanoparticles into the rabbit;#39s eye, with retinoblastoma tumor reduced the size of tumor in the rabbit;#39s eye. Conclusion: It is concluded that the toxicity of gold nanoparticles has a therapeutic effect on Y79 cell line in vitro and in the eye of retinoblastoma tumors in vivo

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