Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran

Authors

Hoda Nouri, Hamid Moghimi,*, Ali KhaleghianAli Khaleghian ORCID
*Corresponding Author: Email: [email protected]

Koomesh:Vol. 22, issue 1; 178-184
Published online:Mar 01, 2020
Article type:Research Article
Received:Dec 01, 2018
Accepted:Jun 01, 2019
How to Cite:Nouri H, Moghimi H, Khaleghian A. Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran. koomesh. 2020;22(1):e153165. doi:

Abstract

Introduction: The use of L-asparaginase as a chemotherapeutic agent is an effective strategy for the treatment of leukemic lymphoblasts. Since the use of bacterial L-asparaginase causes side effects due to hypersensitivity reactions, search for new enzyme sources is one of the active research areas. In this study, the characterization of produced L-asparaginase by indigenous yeast strains was investigated. Material and methods: In this study, 130 yeast strains were evaluated. Remarkably, AG90 isolate with 0.94 IU/ml enzyme production was selected as the superior strain. Results: The molecular identification of the AG90 showed that this strain exhibited a high level of similarity (99%) with Sarocladium sp. and introduced as a Sarocladium sp. AG90. The fermentation broth of the superior strain showed that 86% of the enzyme was produced extracellularly. The Km and Vmax values of the produced enzyme for the L-Asparagine substrate were calculated as 9.74 mM and 19.19 μmol/min, respectively. Discussion: The obtained results in this study can help to introduce a new enzyme with eukaryotic origin to reduce the toxicity, sensitivity and side effects associated of drug consumption.  

Highlights

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Copyright

© 2020, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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