Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran

authors:

avatar Hoda Nouri , avatar Hamid Moghimi , * , avatar Ali Khaleghian ORCID

Koomesh: Vol.22, issue 1; 178-184
published online: March 01, 2020
article type: Research Article
received: December 01, 2018
accepted: June 01, 2019

How To Cite Nouri H, Moghimi H, Khaleghian A. Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran. koomesh. 2020;22(1):e153165. 

Abstract

Introduction: The use of L-asparaginase as a chemotherapeutic agent is an effective strategy for the treatment of leukemic lymphoblasts. Since the use of bacterial L-asparaginase causes side effects due to hypersensitivity reactions, search for new enzyme sources is one of the active research areas. In this study, the characterization of produced L-asparaginase by indigenous yeast strains was investigated. Material and methods: In this study, 130 yeast strains were evaluated. Remarkably, AG90 isolate with 0.94 IU/ml enzyme production was selected as the superior strain. Results: The molecular identification of the AG90 showed that this strain exhibited a high level of similarity (99%) with Sarocladium sp. and introduced as a Sarocladium sp. AG90. The fermentation broth of the superior strain showed that 86% of the enzyme was produced extracellularly. The Km and Vmax values of the produced enzyme for the L-Asparagine substrate were calculated as 9.74 mM and 19.19 μmol/min, respectively. Discussion: The obtained results in this study can help to introduce a new enzyme with eukaryotic origin to reduce the toxicity, sensitivity and side effects associated of drug consumption.  

References

  • 1.

    Wang Y, Qian S, Meng G, Zhang S. Cloning and expression of L-asparaginase gene in Escherichia coli. Appl Biochem Biotechnol 2001; 95: 93-101.

  • 2.

    Abshire T, Pollock B, Billett A, Bradley P, Buchanan G. Weekly polyethylene glycol conjugated (PEG) L-asparaginase (ASP) produces superior induction remission rates in childhood relapsed acute lymphoblastic leukemia (rALL): a Pediatric Oncology Group (POG) study 9310. in Proc Am Soc Clin Oncol 1995; 14: 344.

  • 3.

    Aljewari HS, Nader MI, Alfaisal AH. High efficiency, selectivity against cancer cell line of purified L-Asparaginase from pathogenic Escherichia coli. World Acad Sci Engin Technol 2010; 4: 199-204.

  • 4.

    Souza PM, de Freitas MM, Cardoso SL, Pessoa A, Guerra EN, Magalhaes PO. Optimization and purification of l-asparaginase from fungi: A systematic review. Crit Rev Oncol Hematol 2017; 120: 194-202.

  • 5.

    Vimal A, Kumar A, Biotechnological production and practical application of L-asparaginase enzyme. Biotechnol Genet Eng Rev 2017; 33: 40-61.

  • 6.

    Appel IM, van Kessel-Bakvis C, Stigter R, Pieters R. Influence of two different regimens of concomitant treatment with asparaginase and dexamethasone on hemostasis in childhood acute lymphoblastic leukemia. Leukemia 2007; 21: 2377.

  • 7.

    Ashihara Y, Kono T, Yamazaki S, Inada Y. Modification of E. coli L-asparaginase with polyethylene glycol: disappearance of binding ability to anti-asparaginase serum. Biochem Biophys Res Commun 1978; 83: 385-391.

  • 8.

    Lanvers-Kaminsky C. Asparaginase pharmacology: challenges still to be faced. Cancer Chemother Pharmacol 2017; 79: 439-450.

  • 9.

    Hosamani R, Kaliwal BB. L-asparaginase an anti-tumor agent production by Fusarium equiseti using solid state fermentation. Int J Drug Discover 2011; 3: 88-99.

  • 10.

    Boos J, Werber G, Ahlke E, Schulze-Westhoff P, Nowak-Gttl U, Wrthwein G, et al. Monitoring of asparaginase activity and asparagine levels in children on different asparaginase preparations. Eur J Cancer 1996; 32A: 1544-1550.

  • 11.

    Baskar G, Renganathan S. Optimization of Lasparaginase production by Aspergillus terreus MTCC 1782 using response surface methodology and artificial neural networklinked genetic algorithm. AsiaPacific J Chem Engin 2012; 7: 212-220.

  • 12.

    Batool T, Makky EA, Jalal M, Yusoff MM. A Comprehensive Review on L-Asparaginase and Its Applications. Appl Biochem Biotechnol 2016; 178: 900-923.

  • 13.

    Eijsink VG, Gseidnes S, Borchert TV, van den Burg B. Directed evolution of enzyme stability. Biomolecul Engin 2005; 22: 21-30.

  • 14.

    Chow Y, Ting AS. Endophytic l-asparaginase-producing fungi from plants associated with anticancer properties. J Adv Res 2015; 6: 869-876.

  • 15.

    Deshpande N, Choubey P, Agashe M. Studies on optimization of growth parameters for L-asparaginase production by Streptomyces ginsengisoli. Scientific World Journal 2014; 2014: 895167.

  • 16.

    Erva RR, Goswami AN, Suman P, Vedanabhatla R, Rajulapati SB. Optimization of L-asparaginase production from novel Enterobacter sp., by submerged fermentation using response surface methodology. Prep Biochem Biotechnol 2017; 47: 219-228.

  • 17.

    Sambrook J. Molecular cloning: a laboratory manual. 2001, New York: Cold spring harbor laboratory press.

  • 18.

    Klis FM, de Jong M, Brul S, de Groot PW. Extraction of cell surfaceassociated proteins from living yeast cells. Yeast 2007; 24: 253-258.

  • 19.

    Cachumba JJ, Antunes FA, Peres GF, Brumano LP, Dos Santos JC, Da Silva SS. Current applications and different approaches for microbial l-asparaginase production. Braz J Microbiol 2016; 47: 77-85.

  • 20.

    Kotzia GA, Labrou NE. Cloning, expression and characterisation of Erwinia carotovora L-asparaginase. J Biotechnol 2005; 119: 309-323.

  • 21.

    Shakerimoghadam F, Dejamfekr M, Ghaffari SH, Ahmadian S, Khaleghian A. Arsenic trioxide induction autophagy in human acute promyelocytic leukemia. Koomesh 2018; 20: 764-771.

  • 22.

    Azizi SO, Tarinejad AL. Isolation, cloning and analysis of the glucose transporter gene 7 from Iranian strain of Saccharomyces cerevisiae. Koomesh 2015; 16: 356-365.

  • 23.

    Distasio JA, Niederman RA, Kafkewitz D, Goodman D. Purification and characterization of L-asparaginase with anti-lymphoma activity from Vibrio succinogenes. J Biol Chem 1976; 251: 6929-6933.

  • 24.

    Doriya K, Kumar DS. Isolation and screening of L-asparaginase free of glutaminase and urease from fungal sp. 3 Biotech. 2016; 6: 239.

  • 25.

    El-Bessoumy AA, Sarhan M, Mansour J. Production, isolation, and purification of L-asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation. BMB Reports 2004; 37: 387-393.

  • 26.

    Krishnapura PR, Belur PD, Subramanya S. A critical review on properties and applications of microbial l-asparaginases. Crit Rev Microbiol 2016; 42: 720-737.

  • 27.

    Shrivastava A, Khan AA, Khurshid M, Kalam MA, Jain SK, Singhal PK. Recent developments in L-asparaginase discovery and its potential as anticancer agent. Crit Rev Oncol Hematol 2016; 100: 1-10.

  • 28.

    Albanese ER, Kafkewitz K. Effect of medium composition on the growth and asparaginase production of Vibrio succinogenes. Appl Environ Microbiol 1978; 36: 25-30.

  • 29.

    Kumar NM, Ramasamy R, Manonmani HK. Production and optimization of l-asparaginase from Cladosporium sp. using agricultural residues in solid state fermentation. Industrial Crops Products 2013; 43: 150-158.##.