Interaction between 5-HT6 receptors and acute stress and corticosterone on fear memory reconsolidation in mice

authors:

avatar Rajab Mohammad Rezaei , avatar Reza Pourali-Malabad , avatar Abdolhossein Shiravi , avatar A. li Rashidy-Pour , avatar Abbas Ali Vafaei , *

Koomesh: Vol.22, issue 1; 185-191
published online: March 01, 2020
article type: Research Article
received: January 02, 2019
accepted: July 01, 2019

how to cite: Mohammad Rezaei R, Pourali-Malabad R, Shiravi A, Rashidy-Pour A L, Vafaei A A. Interaction between 5-HT6 receptors and acute stress and corticosterone on fear memory reconsolidation in mice. koomesh. 2020;22(1):e153166. 

Abstract

Introduction: Rrecent studies indicated that serotonergic receptors can be mediate the glucocorticoids’ effects on behavioral procesess. The aim of this study was to determine the role of serotonergic receptor (5-HT6) in the effects of acute stress and corticosterone on fear memory reconsolidation in mice. Materials and Methods: Male adult mice were trained and tested in an inhibitory avoidance task (1mA, 3sec). For assessment memory reconsolidation, immediately after memory reactivation memory (48 hr. after training) animal received corticosterone or exposed to an acute restraint stress (10 min) and also received SC203575 as a 5-HT6 receptor agonist or SB271046 as an antagonist of 5-HT6 receptors simultaneously. Memory retention test was done 5, 8, 10 and 12 days after memory reactivation. Results: The results showed that corticosterone injection or acute stress application after memory reactivation impaired memory reconsolidation in subsequent tests. Co-treatment with both the 5-HT6 agonist SC203575 and antagonist SB271046 inhibited the effects of corticosterone or acute stress on memory reconsolidation. Conclusion: These findings indicated that the 5-HT6 receptors modulate the effects of glucocorticoids or acute stress on fear memory reconsolidation.  

References

  • 1.

    Roozendaal B. Glucocorticoids and the regulation of memory consolidation. Psychoneuroendocrinology 2000; 25: 213-238.

  • 2.

    Murphy D, Costall B, Smythe JW. Regulation of hippocampal theta activity by corticosterone: opposing functions of mineralocorticoid and glucocorticoid receptors. Brain Res Bull 1998; 45: 631-635.

  • 3.

    McGaugh JL. The amygdala modulates the consolidation of memories of emotionally arousing experiences. Annu Rev Neurosci 2004; 27: 1-28.

  • 4.

    Roozendaal B, McGaugh JL. Glucocorticoid receptor agonist and antagonist administration into the basolateral but not central amygdala modulates memory storage. Neurobiol Learn Memory 1997; 67: 176-179.

  • 5.

    Rashidy-Pour A, Vafaei AA, Taherian AA, Miladi-Gorji H, Sadeghi H, Fathollahi Y, Bandegi AR. Verapamil enhances acute stress or glucocorticoid-induced deficits in retrieval of long-term memory in rats. Behav Brain Res 2009; 203: 76-80.

  • 6.

    Roozendaal B, McGaugh JL. Amygdaloid nuclei lesions differentially affect glucocorticoid-induced memory enhancement in an inhibitory avoidance task. Neurobiol Learn Memory 1996; 65: 1-8.

  • 7.

    Yehuda R. Current status of cortisol findings in post-traumatic stress disorder. Psychiatr Clin North Am 2002; 25: 341-368.

  • 8.

    Nikzad S, Vafaei AA, Rashidy-Pour A. Assessment the effects of Metyrapone (as a glucocorticoids synthesis inhibitor) on memory retrieval and reconsolidation in rats. Tabriz Pharmaci Sci 2010; 16: 141-148. (Persian).

  • 9.

    Nikzad S, Vafaei AA, Rashidy-Pour A, Haghighi S. Systemic and intrahippocampal administrations of the glucocorticoid receptor antagonist RU38486 impairs fear memory reconsolidation in rats. Stress 2011; 14: 459-464.

  • 10.

    Vafaei AA, Pakdel R, Nikzad S, Rashidy-Pour A. Effects of mineralocorticoid receptors blockade on fear memory reconsolidation in rats. Basic Clin Neurosci 2011; 2: 58-66.

  • 11.

    Cruz-Morales SE, Garca-Saldvar NL, Gonzlez-Lpez MR, Castillo-Roberto G, Monroy J, Domnguez R. Acute restriction impairs memory in the elevated T-maze (ETM) and modifies serotonergic activity in the dorsolateral striatum. Behav Brain Res 2008; 195: 187-191.

  • 12.

    Jans LA, Blokland A. Influence of chronic mild stress on the behavioural effects of acute tryptophan depletion induced by a gelatin-based mixture. Behav Pharmacol 2008; 19: 706-715.

  • 13.

    Yang YL, Chao PK, Lu KT. Systemic and intra-amygdala administration of glucocorticoid agonist and antagonist modulate extinction of conditioned fear. Neuropsychopharmacology 2006; 31: 912-924.

  • 14.

    Marcos B, Aisa B, Ramrez M. Functional interaction between 5-HT 6 receptors and hypothalamic-pituitary-adrenal axis: Cognitive implications. Neuropharmacology 2008; 54: 708-714.

  • 15.

    Holmes M, Yau J, French K, Seckl J. The effect of adrenalectomy on 5-hydroxytryptamine and corticosteroid receptor subtype messenger RNA expression in rat hippocampus. Neuroscience 1995; 64: 327-337.

  • 16.

    King MV, Marsden CA, Fone KC. A role for the 5-HT 1A, 5-HT 4 and 5-HT 6 receptors in learning and memory. Trends Pharmacol Sci 2008; 29: 482-492.##17] Mokhtari-Zaer A, Ghodrati-Jaldbakhan S, Vafaei AA, Miladi-Gorji H, Akhavan MM, Bandegi AR, Rashidy-Pour A. Effects of voluntary and treadmill exercise on spontaneous withdrawal signs, cognitive deficits and alterations in apoptosisassociated proteins in morphine-dependent rats. Behav Brain Res 2014; 271: 160-170.

  • 17.

    Dehbasi F, Alizadeh N, Rashidy-Pour A, Vafaei AA. Effects of acute stress and corticosterone on fear memory extinction in mice. Koomesh 2012; 13: 375-381. (Persian).

  • 18.

    Donley MP, Schulkin J, Rosen JB. Glucocorticoid receptor antagonism in the basolateral amygdala and ventral hippocampus interferes with long-term memory of contextual fear. Behav Brain Res 2005; 164: 197-205.

  • 19.

    Vafaei AA, Rashidy-Pour A, Taherian AA. Peripheral injection of dexamethasone modulates anxiety related behaviors in mice: an interaction with opioidergic neurons. Pak J Pharm Sci 2008; 21: 285-289.

  • 20.

    Reul JM, van den Bosch FR, de Kloet R. Differential response of type I and type II corticosteroid receptors to changes in plasma steroid level and circadian rhythmicity. Neuroendocrinology 1987; 45: 407-412.

  • 21.

    Beato M, Snchez-Pacheco A. Interaction of steroid hormone receptors with the transcription initiation complex. Endocrine Reviews 1996; 17: 587-609.

  • 22.

    Munck A, Mendel DB, Smith LI, Orti E. Glucocorticoid receptors and actions. Am Rev Respir Dis 1990; 141: S2-10.

  • 23.

    Roozendaal B, Quirarte GL, McGAUGH JL. Stressactivated hormonal systems and the regulation of memory storage. Ann N Y Acad Sci 1997; 821: 247-258.##.