Anticancer effects of Perovskia abrotanoides hydroalcoholic extracts on MKN45 gastric cancer cells

authors:

avatar Mahsa Iraji , avatar Melika Sadegh , avatar Ali khaleghian ORCID , *

Koomesh: Vol.23, issue 2; 291-300
published online: September 01, 2021
article type: issue_documents_importer
received: July 01, 2019
accepted: September 01, 2020

how to cite: Iraji M, Sadegh M, khaleghian A. Anticancer effects of Perovskia abrotanoides hydroalcoholic extracts on MKN45 gastric cancer cells. koomesh. 2021;23(2):e153268. 

Abstract

Introduction: Gastric cancer is the fourth most common cancer worldwide and the second leading cause of death from cancer. Given that existing treatments for it, but the high cost and side effects, have not demonstrated full efficacy and rely on traditional medicine and medicinal plants due to the antioxidant activity of Provskia abrotanoides, this study was performed to investigate the effects of the plant extract in improving gastric cancer. Materials and Methods: In this exprimantal study, Perovskia abrotanoides, collected and after certified specialists from the Center for Applied Science of Jahad Keshavarzi in Semnan, was extracted dry. Human gastric adenocarcinoma cell line MKN 45 was bought from the toxicity of herbal medicine on cancer cells MKN45 were investigated by using the test MTT (dimethylthiazol diphenyl tetrazolium bromide). The antioxidant properties of the hydroalcoholic extracts were evaluated by two in vitro tests, DPPH radical scavenging and reducing power (FRAP). Wound healing and morphological modification were performed in vitro to examine migration and adhesion in the gastric cancer cell line by invert microscopy. Results: The results showed that with increasing concentration of the extract of Perovskia abrotanoides, with a significant relationship, the percentage of remaining living cells of MKN45, had fallen (P˂0.001).At all concentrations of 10, 50 and 100 micrograms per ml of extract, with a significant correlation was equal with the antioxidant properties of 10 micrograms per ml vitamin C (P˂0.001). The the hydroalcoholic extracts showed considerable cytotoxic activity against cancer cell lines (IC50 = 10 μg・mL−1). It was found that the proliferation rate of MKN-45 cells decreased after treatment with the extracts in a dosedependent way. Conclusion: The results of this study showed that the Perovskia abrotanoides extract had high cytotoxic effect on gastric cancer cells (MKN45) and the percentage of lethality, with the passage of time and with increased concentration had risen.  

References

  • 1.

    Antognoli S, Chiorrini S, Cascinu S. Chemotherapy for advanced gastric cancer: across the years for a standard of care. Expert Opin Pharmacother 2007; 8: 797-808.##https://doi.org/10.1517/14656566.8.6.797.

  • 2.

    Roshanaei G, Kazemnejad A, Sedighi S. Postoperative survival estimation of gastric cancer patients in cancer institute of Tehran, Imam Khomeini hospital and its relative factors. Avicenna J Clin Med 2010; 17: 13-18. (Persian).

  • 3.

    Ghahreman A. Iranian naturelle colorfull Flora. La Science faculty, Tehran univ, Iran: Research Institute of Forests and Rangelands 1986; 9: 1059-1060. (Persian).

  • 4.

    Beikmohammadi M. The evaluation of medicinal properties of Perovskia abrotanoides Karel. Middle East J Sci Res 2012; 11: 189-193. (Persian).##.

  • 5.

    Fallah Huseini H, Fakhrzadeh H, Larijani B, Shikh Samani A. Review of anti-diabetic medicinal plant used in traditional medicine. J Medicin Plants 2006; 1: 1-8. (Persian).##.

  • 6.

    Arabi F, Moharramipour S, Sefidkon F. Chemical composition and insecticidal activity of essential oil from Perovskia abrotanoides (Lamiaceae) against Sitophilus oryzae (Coleoptera: Curculionidae) and Tribolium castaneum (Coleoptera: Tenebrionidae). Int J Tropical Insect Sci 2008; 28: 144-150. (Persian).

  • 7.

    Organization WH. Preventing chronic diseases: a vital investment: WHO global report: World Health Organization; 2005.##.

  • 8.

    Geryani MA, Mahdian D, Mousavi SH, Hosseini A. Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines. Avicenna J Phytomed 2016; 6: 410.##.

  • 9.

    Soni A, Krishnamurthy R. Plants-the next generation treatment of leukemia. Indian J Plant Sci 2013; 2: 117-125.##.

  • 10.

    Nigusse Z, Wondifraw W, Abate S. Screening of aloe otallensis exudate and its effect on Leishmania aethiopica. Pharm Anal Acta 2016; 7.

  • 11.

    Zaker A, Sykora C, Gssnitzer F, Abrishamchi P, Asili J, Mousavi SH, Wawrosch C. Effects of some elicitors on tanshinone production in adventitious root cultures of Perovskia abrotanoides Karel. Indust Crops Products 2015; 67: 97-102. (Persian).

  • 12.

    Hu T, Cho C. Potential applications of tanshinones in gastrointestinal and hepatic diseases. J Biomol Res Ther 2013; 2: 2.

  • 13.

    Liang S, Guo R, Zhang Z, Liu D, Xu H, Xu Z, Wang X, Yang L. Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth. Oncol Rep 2013; 29: 2422-2430.

  • 14.

    Zamanian-Azodi M, Rezaie-Tavirani M, Heydari-Kashal S, Kalantari S, Dailian S, Zali H. Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract. Gastroenterol Hepatol Bed Bench 2012; 5: 35-42.

  • 15.

    Hung KF, Hsu CP, Chiang JH, Lin HJ, Kuo YT, Sun MF, Yen HR. Complementary Chinese herbal medicine therapy improves survival of patients with gastric cancer in Taiwan: a nationwide retrospective matched-cohort study. J Ethnopharmacology 2017; 199: 168-174.##https://doi.org/10.1016/j.jep.2017.02.004.

  • 16.

    Marcelo G, Ariana-Machado J, Enea M, Carmo H, Rodrguez-Gonzlez B, Luis Capelo J, et al. Toxicological evaluation of luminescent silica nanoparticles as new drug nanocarriers in different cancer cell lines. Materials 2018; 11: 1310.##https://doi.org/10.3390/ma11081310.

  • 17.

    Morteza-Semnani K, Ghanbarimasir Z. A review on traditional uses, phytochemistry and pharmacological activities of the genus ballota. J Ethnopharmacology 2019; 233: 197-217.##https://doi.org/10.1016/j.jep.2018.12.001.

  • 18.

    Akar B, Akar Z, Sahin B. Identification of antioxidant activity by different methods of a freshwater alga (Microspora Sp.) collected from a high mountain lake. Hitt J Sci Engin 2019; 6: 25-29.

  • 19.

    Ghaderi S, Ebrahimi SN, Ahadi H, Moghadam SE, Mirjalili MH. In vitro propagation and phytochemical assessment of Perovskia abrotanoides Karel. (Lamiaceae)-A medicinally important source of phenolic compounds. Biocatal Agricult Biotechnol 2019; 19: 101-113.

  • 20.

    Fraternale D, Sosa S, Ricci D, Genovese S, Messina F, Tomasini S, Montanari F, Marcotullio MC. Anti-inflammatory, antioxidant and antifungal furanosesquiterpenoids isolated from Commiphora erythraea (Ehrenb.) Engl Resin Fitoterapia 2011; 82: 654-661.

  • 21.

    Abdul Baqi S, Tareen RB, Mengal A, Khan N, Behlil F, Achakzai AK, et al. 35. Determination of antioxidants in two medicinally important plants, Haloxylon griffithii and Convolvulus leiocalycinus, of Balochistan. Pure Appl Biol 2018; 7: 296-308.

  • 22.

    Sim Y, Shin S. Antibacterial activities of the essential oil from the leaves and rhizomes of Cnidium officinale Makino. J Essent Oil Res 2014; 26: 452-457.

  • 23.

    Zhu G, Wong B, Eggo M, Ching C, Yuen S, Chan E, Lai K, Lam S. Non-steroidal anti-inflammatory drug-induced apoptosis in gastric cancer cells is blocked by protein kinase C activation through inhibition of c-myc. Br J Cancer 1999; 79: 393-400.

  • 24.

    Zhou XM, Wong BC, Fan XM, Zhang HB, Lin MC, Kung HF, et al. Non-steroidal anti-inflammatory drugs induce apoptosis in gastric cancer cells through up-regulation of bax and bak. Carcinogenesis 2001; 22: 1393-1397.

  • 25.

    Liu ZL, Chu SS, Jiang CH, Hou J, Liu QZ, Jiang GH. Composition and insecticidal activity of the essential oil of lindera aggregata root tubers against sitophilus zeamais and tribolium castaneum. J Essent Oil Bearin Plants 2016; 19: 727-733.

  • 26.

    Zheng YX, Yang M, Rong TT, Yuan XL, Ma YH, Wang ZH, et al. CD74 and macrophage migration inhibitory factor as therapeutic targets in gastric cancer. World J Gastroenterol 2012; 18: 2253.

  • 27.

    Roodsari MR, Zamanian-Azodi M, Salimpour F. Herbal remedies and medicine; introducing some Iranian plants. J Paramed Sci 2013; 4: 2008-4978.##.

  • 28.

    Nejad SK, Jaimand K, Monfared A, Akbarzadeh M. Study of the chemical composition of essential oils of Perovskia abrotanoides karel at the different stage and distillation by gas chromatography. J Med Plants By-Products 2013; 2: 139-142. (Persian).##.

  • 29.

    Daz C, Quesada S, Brenes O, Aguilar G, Ciccio JF. Chemical composition of Schinus molle essential oil and its cytotoxic activity on tumour cell lines. Nat Prod Res 2008; 22: 1521-1534.

  • 30.

    Roh J, Shin S. Biological activities of essential oils from Angelica tenuissima Nakai. Nat Prod Sci 2013; 19: 297-302.##.

  • 31.

    Sairafianpour M, Christensen J, Strk D, Budnik BA, Kharazmi A, Bagherzadeh K, Jaroszewski JW. Leishmanicidal, antiplasmodial, and cytotoxic activity of novel diterpenoid 1, 2-quinones from Perovskia abrotanoides: new source of tanshinones. J Nat Prod 2001; 64: 1398-1403.

  • 32.

    Li S, Zhaohuan L, Guangshun Z, Guanhua X, Guangji Z. Diterpenoid tanshinones, the extract from Danshen (Radix Salviae Miltiorrhizae) induced apoptosis in nine human cancer cell lines. J Tradit Chin Med 2016; 36: 514-521.

  • 33.

    Chen GY, Shu YC, Chuang DY, Wang YC. Inflammatory and apoptotic regulatory activity of tanshinone IIA in Helicobacter pylori-infected cells. Am J Chin Med 2016; 44: 1187-1206.

  • 34.

    Zaker A, Asili J, Abrishamchi P, Tayarani-Najaran Z, Mousavi SH. Cytotoxic and apoptotic effects of root extract and tanshinones isolated from Perovskiaabrotanoides Kar. Iran J Basic Med Sci 2017; 20: 1377.

  • 35.

    Ashraf SN, Zubair M, Rizwan K, Tareen RB, Rasool N, Zia-Ul-Haq M, Ercisli S. Compositional studies and Biological activities of Perovskia abrotanoides Kar. oils. Biol Res 2014; 47: 12.