Antinociceptive and anti-inflammatory, and acute toxicity effects of Asparagus persicus essential oil in male mice

authors:

avatar Ali reza fallahzadeh , avatar saeed mohammadi , *

Koomesh: Vol.23, issue 4; 474-481
published online: August 03, 2021
article type: Research Article
received: July 09, 2020
accepted: January 03, 2021

how to cite: fallahzadeh A R, mohammadi S. Antinociceptive and anti-inflammatory, and acute toxicity effects of Asparagus persicus essential oil in male mice. koomesh. 2021;23(4):e153276. 

Abstract

Introduction: In Iranian traditional medicine, Asparagus persicus has been used for treating rheumatic pain and inflammation. The aim of this study was to evaluate the analgesic and anti-inflammatory as well as acute toxicity effects of Asparagus persicus root essential oil (APEO) in male mice. Materials and Methods: Male adult mice were used. In pain assessment tests (writhing, tail-flick, and formalin tests), animals divided to the six groups: control/vehicle (Tween 80+distilled water), three groups treated with the APEO (100, 200, and 400 mg/kg, gavage/oral treating), morphine (i.p.), and naloxone (i.p.) plus APEO (400 mg/kg). Moreover, inflammation test (xylene and carrageenan tests), animals divided to five groups: control, three groups of APEO (orally), and dexamethasone (i.p.). For toxicity tests, the animals were divided to the six groups. Results: Results showed that APEO at a dose of 400 mg/kg in writhing and tailflick tests induced an antinociceptive effect as compared with the control (P

References

  • 1.

    Spisak T, Kincses B, Schlitt F, Zunhammer M, Schmidt-Wilcke T, Kincses ZT, Bingel U. Pain-free resting-state functional brain connectivity predicts individual pain sensitivity. Nat Commun 2020; 11: 1-12. https://doi.org/10.1038/s41467-019-13785-z PMid:31924769 PMCid:PMC6954277.

  • 2.

    Liao Y, Zhao J, Bulek K, Tang F, Chen X, Cai G, et al. Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis. Nat Commun 2020; 11: 1-15. https://doi.org/10.1038/s41467-020-14698-y PMid:32060280 PMCid:PMC7021685.

  • 3.

    Karimy JK, Reeves BC, Damisah E, Duy PQ, Antwi P, David W, et al. Inflammation in acquired hydrocephalus: pathogenic mechanisms and therapeutic targets. Nat Rev Neurol 2020; 1-12. https://doi.org/10.1038/s41582-020-0321-y PMid:32152460 PMCid:PMC7375440.

  • 4.

    Asgari Nematian M, Yaghmaei P, Mohammadi S. Assessment of the antinociceptive, antiinflammatory and acute toxicity effects of Ducrosia anethifolia essential oil in mice. Sci J Kurdistan Univ Med Sci 2017; 22: 74-84. (Persian).

  • 5.

    Bussmann RW, Batsatsashvili K, Kikvidze Z. Asparagus persicus Baker Asparagus sp. A sparagaceae. Ethnobotany of the Mountain Regions of Central Asia and Altai 2020: 1-6. https://doi.org/10.1007/978-3-319-77087-1_24-1 PMCid:PMC7525228.

  • 6.

    Mousavizadeh SJ, Hassandokht MR, Kashi A. Multivariate analysis of edible Asparagus species in Iran by morphological characters. Euphytica 2015; 206: 445-457. https://doi.org/10.1007/s10681-015-1508-y.

  • 7.

    Kargar JH, Karimi JH, Kargar JZ, Khabbaz KZ. The effect of the aqueous root extract of edible asparagus native to iran on serum progesterone and lh levels in adult female rats. 2015. (Persian).

  • 8.

    Negi J, Singh P, Joshi G, Rawat M, Bisht V. Chemical constituents of Asparagus. Pharmacogn Rev 2010; 4: 215. https://doi.org/10.4103/0973-7847.70921 PMid:22228964 PMCid:PMC3249924.

  • 9.

    Nishizawa M, Kano M, Okuyama T, Okumura T, Ikeya Y. Anti-inflammatory effects of enzyme-treated asparagus extract and its constituents in hepatocytes. Funct Foods Health Dise 2016; 6: 91-109. https://doi.org/10.31989/ffhd.v6i2.228.

  • 10.

    Nwafor PA, Okwuasaba F. Anti-nociceptive and anti-inflammatory effects of methanolic extract of Asparagus pubescens root in rodents. J Ethnopharmacol 2003; 84: 125-129. https://doi.org/10.1016/S0378-8741(02)00213-1.

  • 11.

    Mittal S, Dixit PK. In-vivo anti-inflammatory and anti-arthritic activity of Asparagus racemosus roots. Int J Pharmace Sci Res 2013; 4: 2652. https://doi.org/10.7897/2230-8407.04433.

  • 12.

    Golshani Y, Mohammadi S. Antidepressant and anxiolytic effects of Rhus Coriaria in male rats. KAUMS J (FEYZ) 2019; 23: 476-484. (Persian).

  • 13.

    Golshani Y, Mohammadi S. Antinociceptive and acute toxicity effects of erigeron acer L. In adult male rats. J Med Plants 2017; 2: 85-93. (Persian).

  • 14.

    Self I, Grubb T. Physiology of pain. BSAVA Guide to Pain Management in Small Animal Practice: BSAVA Library; 2019; p: 3-13. https://doi.org/10.22233/9781910443453.2##.

  • 15.

    Sosa S, Pelin M, Cavion F, Herv F, Hess P, Tubaro A. Acute oral toxicity of pinnatoxin G in mice. Toxins 2020; 12: 87. https://doi.org/10.3390/toxins12020087 PMid:32012834 PMCid:PMC7076786##.

  • 16.

    Tekulu GH, Hiluf T, Brhanu H, Araya EM, Bitew H, Haile T. Anti-inflammatory and anti-nociceptive property of Capparis tomentosa Lam. root extracts. J Ethnopharmacol 2020; 253: 112654. https://doi.org/10.1016/j.jep.2020.112654 PMid:32035216##.

  • 17.

    Yuan HL, Zhao Yl, Ding CF, Zhu PF, Jin Q, Liu YP, et al. Anti-inflammatory and antinociceptive effects of Curcuma kwangsiensis and its bioactive terpenoids in vivo and in vitro. J Ethnopharmacol 2020; 259: 112935. https://doi.org/10.1016/j.jep.2020.112935 PMid:32387235##.

  • 18.

    Gawade S. Acetic acid induced painful endogenous infliction in writhing test on mice. J Pharmacol Pharmacother 2012; 3: 348. https://doi.org/10.4103/0976-500X.103699 PMid:23326113 PMCid:PMC3543562##.

  • 19.

    Zarei M, Mohammadi S, Komaki A. Antinociceptive activity of Inula britannica L. and patuletin: In vivo and possible mechanisms studies. J Ethnopharmacology 2018; 219: 351-358. https://doi.org/10.1016/j.jep.2018.03.021 PMid:29567278##.

  • 20.

    Taherian AA, Sameni HR, Sharifi S, Taherian MH. Effects of hydroalchoholic extract of Iranian Propolis on acute, chronic and visceral pain in mice. Koomesh 2020; 22: 170-177. (Persian). https://doi.org/10.29252/koomesh.22.1.170##.

  • 21.

    Mahmoodi M, Mohammadi S, Yavari A. Antinociceptive effect of hydro-alcoholic extract of biophytum sensivitum leaf on adult male rat. J Babol Univ Med Sci 2014; 16: 31-37. (Persian).##.

  • 22.

    McMahon SB, Koltzenburg M, Tracey I, Turk D. Wall & melzack's textbook of pain e-book: Elsevier Health Sciences; 2013; 192-193.##.

  • 23.

    Golshani Y, Mohammadi S. Evaluation of antinociceptive effect of methanolic extract of Lallemantia iberica in adult male rats. Armaghane Danesh 2015; 19: 1058-1068. (Persian).##.

  • 24.

    Mahmoudi M, Mohammadi S, Shahidi S. Antinociceptive effect of hydroalcoholic leaf extract of Hedera helix in male rat. Avicenna J Clin Med 2013; 20: 119-125.##.

  • 25.

    Fallahzadeh AR, Mohammadi S. Assessment of the antinociceptive, anti-inflammatory, and acute toxicity effects of solanum dulcamara essential oil in male mice. J Babol Univ Med Sci 2020; 22: 162-168. (Persian)##.

  • 26.

    Sharifi SM, Eidi A, Haeri RS. Anti-Inflammatory effect of glycyrrhiza glabra ethanolic extract in adultmale NMRI mice. 2018; 11: 15-25. (Persian).##.

  • 27.

    Asgari NM, Mohammadi S. The analgesic effect of Echinophora platyloba hydroalcoholic extract in male rats. 2016; 18: 31-37. (Persian).##.

  • 28.

    Hassan H, Ahmadu A, Hassan A. Analgesic and anti-inflammatory activities of Asparagus africanus root extract. Afr J Tradit Complement Altern Med 2008; 5: 27-31. https://doi.org/10.4314/ajtcam.v5i1.31252 PMid:20162051 PMCid:PMC2816601##.

  • 29.

    Iqbal M, Bibi Y, Raja NI, Ejaz M, Hussain M, Yasmeen F, et al. Review on therapeutic and pharmaceutically important medicinal plant Asparagus officinalis L. J Plant Biochem Physiol 2017; 5: 2. https://doi.org/10.4172/2329-9029.1000180##.

  • 30.

    Mahmoodi M, Mohammadi S, Enayati F. Evaluation of the antinociceptive effect of hydroalcoholic extract of Potentilla reptans L. in the adult male rat. SSU J 2016; 24: 201-210. (Persian).##.

  • 31.

    Vanegas H, Barbaro NM, Fields HL. Tail-flick related activity in medullospinal neurons. Brain Res 1984; 321: 135-141. https://doi.org/10.1016/0006-8993(84)90689-9##.

  • 32.

    Xiang-Hui J, Gui-Ping Z, Jun OL, Chao-Wen S. An efficient system for the production of the medicinally important plant: Asparagus cochinchinensis (Lour.) Merr. Afr J Biotechnol 2010; 9: 6207-6212.

  • 33.

    Wootton M. Morphine is not the only analgesic in palliative care: literature review. J Adv Nurs 2004; 45: 527-532. https://doi.org/10.1046/j.1365-2648.2003.02936.x PMid:15009356##.

  • 34.

    Karbab A, Mokhnache K, Ouhida S, Charef N, Djabi F, Arrar L, Mubarak MS. Anti-inflammatory, analgesic activity, and toxicity of Pituranthos scoparius stem extract: An ethnopharmacological study in rat and mouse models. J Ethnopharmacol 2020; 258: 112936. https://doi.org/10.1016/j.jep.2020.112936 PMid:32376367##.

  • 35.

    Choo BK, Yoon T, Cheon MS, Lee HW, Lee AY, Kim HK. Anti-inflammatory effects of Asparagus cochinchinensis extract in acute and chronic cutaneous inflammation. J Ethnopharmacol 2009; 121: 28-34. https://doi.org/10.1016/j.jep.2008.07.006 PMid:18691647##.

  • 36.

    Battu G, Kumar B. Anti-inflammatory activity of leaf extract of Asparagus racemosus Willd. Int J Chem Sci 2010; 8: 1329-1338.##.

  • 37.

    Yang WS, Ko J, Kim E, Kim JH, Park JG, Sung NY, et al. 21-O-Angeloyltheasapogenol E3, a novel triterpenoid saponin from the seeds of tea plants, inhibits macrophage-mediated inflammatory responses in a NF-B-dependent manner. Mediators Inflamm 2014; 2014: 658351. https://doi.org/10.1155/2014/658351 PMid:25477714 PMCid:PMC4245502##.

  • 38.

    Han Y, Zhang X, Qi R, Li X, Gao Y, Zou Z, Cai R, Qi Y. Lucyoside B, a triterpenoid saponin from Luffa cylindrica, inhibits the production of inflammatory mediators via both nuclear factor-B and activator protein-1 pathways in activated macrophages. J Funct Foods 2020; 69: 103941. https://doi.org/10.1016/j.jff.2020.103941##.

  • 39.

    Ahmadimoghaddam D, Sadeghian R, Ranjbar A, Izadidastenaei Z, Mohammadi S. Antinociceptive activity of Cnicus benedictus L. leaf extract: a mechanistic evaluation. Res Pharm Sci 2020; 1: 463-472. https://doi.org/10.4103/1735-5362.297849 PMid:33628288 PMCid:PMC7879793##.

  • 40.

    Maleki SJ, Crespo JF, Cabanillas B. Anti-inflammatory effects of flavonoids. Food Chem 2019; 299: 125124. https://doi.org/10.1016/j.foodchem.2019.125124 PMid:31288163##.

  • 41.

    Ferraz CR, Carvalho TT, Manchope MF, Artero NA, Rasquel-Oliveira FS, Fattori V, et al. Therapeutic potential of flavonoids in pain and inflammation: mechanisms of action, pre-clinical and clinical data, and pharmaceutical development. Molecules 2020; 25: 762. https://doi.org/10.3390/molecules25030762 PMid:32050623 PMCid:PMC7037709##.

  • 42.

    Nwafor PA, Jacks T, Longe O. Acute toxicity study of Methanolic extract of Asparagus pubescens root in rats. Afr J Biomed Res 2004; 7: 9-15. https://doi.org/10.4314/ajbr.v7i1.54060.