Effect of vitamin D administration on blood oxidative stress factors in university students: A randomized double-blind clinical trial

authors:

avatar Fatemeh Dabaghzadeh ORCID , avatar Maryam Rahimpour , avatar Somayyeh Karami-Mohajeri , *


how to cite: Dabaghzadeh F, Rahimpour M, Karami-Mohajeri S. Effect of vitamin D administration on blood oxidative stress factors in university students: A randomized double-blind clinical trial. koomesh. 2021;23(6):e153304. 

Abstract

Introduction: Vitamin D deficiency is widespread in the world and is prevalent in Muslim countries despite sufficient sunlight. Vitamin D deficiency disrupts mitochondrial function and enhances oxidative stress and leads to common diseases such as metabolic disorders. This study aimed to evaluate the efficacy of vitamin D supplementation on oxidative stress factors in students. Materials and Methods: This study was a randomized double-blind clinical trial. University students were randomly divided into two groups of control and treatment with a sample size of 26 and 25 in each group, respectively. Students received two pearls of 50,000 unit vitamin D or placebo at time zero and after 4 weeks. Before the beginning of the study and after 8 weeks, oxidative stress biomarkers including the total antioxidant capacity of plasma and lipid peroxidation were measured in the blood samples. Results: At the beginning of the study, there was no significant difference between the treatment group and placebo in the mean of vitamin D, but after the administration of vitamin D, this difference became significant. Vitamin D significantly increased the total antioxidant capacity (P=0.019) and decreased the lipid peroxidation (P=0.004) compared with the placebo group. Conclusion: The results of this study show the effect of monthly administration of 50,000 units of vitamin D on reducing oxidative stress in students.

References

  • 1.

    DeLuca HF. Overview of general physiologic features and functions of vitamin D. Am J Clin Nutr 2004; 80: 1689S-1696S. https://doi.org/10.1093/ajcn/80.6.1689S PMid:15585789.

  • 2.

    Gunton JE, Girgis CM. Vitamin D and muscle. Bone Rep 2018; 8: 163-167. https://doi.org/10.1016/j.bonr.2018.04.004 PMid:29963601 PMCid:PMC6021354.

  • 3.

    Holick MF. Vitamin D status: measurement, interpretation, and clinical application. Ann Epidemiol 2009; 19: 73-78. https://doi.org/10.1016/j.annepidem.2007.12.001 PMid:18329892 PMCid:PMC2665033.

  • 4.

    Holick MF. The vitamin D epidemic and its health consequences. J Nutr 2005; 135: 2739S-2748S. https://doi.org/10.1093/jn/135.11.2739S PMid:16251641.

  • 5.

    Zittermann A. Vitamin D and disease prevention with special reference to cardiovascular disease. Prog Biophys Mol Biol 2006; 92: 39-48. https://doi.org/10.1016/j.pbiomolbio.2006.02.001 PMid:16600341.

  • 6.

    Sepehrmanesh Z, Kolahdooz F, Abedi F, Mazroii N, Assarian A, Asemi Z, et al. Vitamin D supplementation affects the beck depression inventory, insulin resistance, and biomarkers of oxidative stress in patients with major depressive disorder: a randomized, controlled clinical trial. J Nutr 2016; 146: 243-248. https://doi.org/10.3945/jn.115.218883 PMid:26609167.

  • 7.

    Razavi M, Jamilian M, Karamali M, Bahmani F, Aghadavod E, Asemi Z. The effects of vitamin DK-calcium co-supplementation on endocrine, inflammation, and oxidative stress biomarkers in vitamin D-deficient women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial. Horm Metab Res 2016; 48: 446-451. https://doi.org/10.1055/s-0042-104060 PMid:27050252.

  • 8.

    Polachini CR, Spanevello RM, Zanini D, Baldissarelli J, Pereira LB, Schetinger MR, et al. Evaluation of delta-aminolevulinic dehydratase activity, oxidative stress biomarkers, and vitamin D levels in patients with multiple sclerosis. Neurotox Res 2016; 29: 230-242. https://doi.org/10.1007/s12640-015-9584-2 PMid:26690779.

  • 9.

    Anglin RE, Samaan Z, Walter SD, McDonald SD. Vitamin D deficiency and depression in adults: systematic review and meta-analysis. Br J Psychiatry 2013; 202: 100-107. https://doi.org/10.1192/bjp.bp.111.106666 PMid:23377209.

  • 10.

    Dong JY, Zhang W, Chen JJ, Zhang ZL, Han SF, Qin LQ. Vitamin D intake and risk of type 1 diabetes: a meta-analysis of observational studies. Nutrients 2013; 5: 3551-3562. https://doi.org/10.3390/nu5093551 PMid:24036529 PMCid:PMC3798920.

  • 11.

    Mazahery H, Camargo CA, Conlon C, Beck KL, Kruger MC, von Hurst PR. Vitamin D and autism spectrum disorder: a literature review. Nutrients 2016; 8: 236. https://doi.org/10.3390/nu8040236 PMid:27110819 PMCid:PMC4848704.

  • 12.

    Oliveira SR, Simo AN, Alfieri DF, Flauzino T, Kallaur AP, Mezzaroba L, et al. Vitamin D deficiency is associated with disability and disease progression in multiple sclerosis patients independently of oxidative and nitrosative stress. J Neurol Sci 2017; 381: 213-219. https://doi.org/10.1016/j.jns.2017.07.046##PMid:28991684.

  • 13.

    Walker VP, Modlin RL. The vitamin D connection to pediatric infections and immune function. Pediatr Res 2009; 65: 106R-113R. https://doi.org/10.1203/PDR.0b013e31819dba91 PMid:19190532 PMCid:PMC2925470.

  • 14.

    Bahnam B, Semnani V, Hadadnia F, Mirmohammadkhani M. Vitamin D serum levels in nurses in Semnan educational hospitals and its association with depression. Koomesh 2016; 17: 313-322. (Persian).

  • 15.

    Semnani V, Farhidzadeh E, Mirmohammadkhani M, Ghahremanfard F. Investigation of blood levels of vitamin D in women with breast cancer and its correlation with prognostic markers. Koomesh 2017; 19: 735-741. (Persian).

  • 16.

    Sepidarkish M, Farsi F, Akbari-Fakhrabadi M, Namazi N, Almasi-Hashiani A, Maleki Hagiagha A, et al. The effect of vitamin D supplementation on oxidative stress parameters: A systematic review and meta-analysis of clinical trials. Pharmacol Res 2019; 139: 141-152. https://doi.org/10.1016/j.phrs.2018.11.011 PMid:30447293.

  • 17.

    Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for Human health. Oxid Med Cell Longev 2017; 2017: 8416763. https://doi.org/10.1155/2017/8416763 PMid:28819546 PMCid:PMC5551541.

  • 18.

    Belenguer-Varea A, Tarazona-Santabalbina FJ, Avellana-Zaragoza JA, Martinez-Reig M, Mas-Bargues C, Ingles M. Oxidative stress and exceptional human longevity: Systematic review. Free Radic Biol Med 2020; 149: 51-63. https://doi.org/10.1016/j.freeradbiomed.2019.09.019 PMid:31550529.

  • 19.

    Alkadi H. A review on free radicals and antioxidants. Infect Disord Drug Targets 2020; 20: 16-26. https://doi.org/10.2174/1871526518666180628124323 PMid:29952268.

  • 20.

    Ilahi M, Armas LA, Heaney RP. Pharmacokinetics of a single, large dose of cholecalciferol. Am J Clin Nutr 2008; 87: 688-691. https://doi.org/10.1093/ajcn/87.3.688 PMid:18326608.

  • 21.

    Heidari H, Amani R, Feizi A, Askari G, Kohan S, Tavasoli P. Vitamin D supplementation for premenstrual syndrome-related inflammation and antioxidant markers in students with vitamin D deficient: a randomized clinical trial. Sci Rep 2019; 9: 14939. https://doi.org/10.1038/s41598-019-51498-x PMid:31624297 PMCid:PMC6797739.

  • 22.

    Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 1996; 239: 70-76. https://doi.org/10.1006/abio.1996.0292 PMid:8660627.

  • 23.

    Zeb A, Ullah F. A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. J Anal Methods Chem 2016; 2016: 9412767. https://doi.org/10.1155/2016/9412767 PMid:27123360 PMCid:PMC4830699.

  • 24.

    Kruger NJ. The Bradford method for protein quantitation. In: Walker JM, editor. The Protein Protocols Handbook. Totowa, NJ: Humana Press; 2009; p: 17-24. https://doi.org/10.1007/978-1-59745-198-7_4.

  • 25.

    Bischoff-Ferrari HA, Giovannucci E, Willett WC, Dietrich T, Dawson-Hughes B. Estimation of optimal serum concentrations of 25-hydroxyvitamin D for multiple health outcomes. Am J Clin Nutr 2006; 84: 18-28. https://doi.org/10.1093/ajcn/84.1.18 PMid:16825677.

  • 26.

    Palacios C, Gonzalez L. Is vitamin D deficiency a major global public health problem? J Steroid Biochem Mol Biol 2014; 144: 138-145. https://doi.org/10.1016/j.jsbmb.2013.11.003 PMid:24239505 PMCid:PMC4018438.

  • 27.

    Hovsepian S, Amini M, Aminorroaya A, Amini P, Iraj B. Prevalence of vitamin D deficiency among adult population of Isfahan City, Iran. J Health Popul Nutr 2011; 29: 149. https://doi.org/10.3329/jhpn.v29i2.7857 PMid:21608424 PMCid:PMC3126987.

  • 28.

    Nakhaee S, Ali Yaghoubi M, Zarban A, Amirabadizadeh A, Faghihi V, Yoosef Javadmoosavi S, et al. Vitamin D deficiency and its associated risk factors in normal adult population of Birjand, Iran. Clin Nutr ESPEN 2019; 32: 113-117. https://doi.org/10.1016/j.clnesp.2019.04.002 PMid:31221275.

  • 29.

    Esmaeili SA, Mohammadian S, Radbakhsh S, Momtazi-Borojeni AA, Kheirmand Parizi P, Atabati H, et al. Evaluation of vitamin D3 deficiency: A population-based study in northeastern Iran. J Cell Biochem 2019; 120: 10337-10341. https://doi.org/10.1002/jcb.28317 PMid:30556194.

  • 30.

    Toopchizadeh V, Barzegar M, Masoumi S, Jahanjoo F. Prevalence of Vitamin D deficiency and associated risk factors in cerebral palsy a study in North-West of Iran. Iran J Child Neurol 2018; 12: 25-32.

  • 31.

    Ramezani M, Sadeghi M. The prevalence of Vitamin D deficiency in adults in Kermanshah, Western Iran. Iran J Public Health 2018; 47: 299-300. (Persian).

  • 32.

    Saki F, Dabbaghmanesh MH, Omrani GR, Bakhshayeshkaram M. Vitamin D deficiency and its associated risk factors in children and adolescents in southern Iran. Public Health Nutr 2017; 20: 1851-1856. https://doi.org/10.1017/S1368980015001925 PMid:26051113.

  • 33.

    Pirdehghan A, Vakili M, Dehghan R, Zare F. High prevalence of Vitamin D deficiency and adverse pregnancy outcomes in Yazd, a central province of Iran. J Reprod Infertil 2016; 17: 34-38.

  • 34.

    Faghih S, Abdolahzadeh M, Mohammadi M, Hasanzadeh J. Prevalence of vitamin d deficiency and its related factors among university students in shiraz, iran. Int J Prev Med 2014; 5: 796. (Persian).

  • 35.

    Bhat M, Ismail A. Vitamin D treatment protects against and reverses oxidative stress induced muscle proteolysis. J Steroid Biochem Mol Biol 2015; 152: 171-179. https://doi.org/10.1016/j.jsbmb.2015.05.012 PMid:26047554.

  • 36.

    Zhang HQ, Teng JH, Li Y, Li XX, He YH, He X, et al. Vitamin D status and its association with adiposity and oxidative stress in schoolchildren. Nutrition 2014; 30: 1040-1044. https://doi.org/10.1016/j.nut.2014.02.024 PMid:25102819.

  • 37.

    Dzik K, Skrobot W, Flis DJ, Karnia M, Libionka W, Kloc W, et al. Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain. Eur J Appl Physiol 2018; 118: 143-151. https://doi.org/10.1007/s00421-017-3755-1 PMid:29143122.

  • 38.

    Jungert A, Neuhuser-Berthold M. Cross-sectional and longitudinal associations between serum 25 hydroxyvitamin D and anti-oxidative status in older adults. Exp Gerontol 2018; 110: 291-297. https://doi.org/10.1016/j.exger.2018.06.024 PMid:29953952.

  • 39.

    Boaventura BC, Cembranel F. Chapter 35 - Protective effect of vitamin D on oxidative stress in elderly people. In: Preedy VR, Patel VB, editors. Aging (Second Edition): Academic Press; 2020; p: 337-343. https://doi.org/10.1016/B978-0-12-818698-5.00036-5.

  • 40.

    Tohari AM, Zhou X, Shu X. Protection against oxidative stress by vitamin D in cone cells. Cell Biochem Funct 2016; 34: 82-94. https://doi.org/10.1002/cbf.3167 PMid:26890033.

  • 41.

    Samimi M, Kashi M, Foroozanfard F, Karamali M, Bahmani F, Asemi Z, et al. The effects of vitamin D plus calcium supplementation on metabolic profiles, biomarkers of inflammation, oxidative stress and pregnancy outcomes in pregnant women at risk for preeclampsia. J Hum Nutr Diet 2016; 29: 505-515. https://doi.org/10.1111/jhn.12339 PMid:26467311.

  • 42.

    Gargari BP, Tabrizi FP, Sadien B, Jafarabadi MA, Farzadi L. Vitamin D status is related to oxidative stress but not high-sensitive C-reactive protein in women with pre-eclampsia. Gynecol Obstet Invest 2016; 81: 308-314. https://doi.org/10.1159/000441781 PMid:26587898.

  • 43.

    Berridge MJ. Vitamin D: a custodian of cell signalling stability in health and disease. Biochem Soc Trans 2015; 43: 349-358. https://doi.org/10.1042/BST20140279 PMid:26009175.

  • 44.

    Berridge MJ. Vitamin D cell signalling in health and disease. Biochem Biophys Res Commun 2015; 460: 53-71. https://doi.org/10.1016/j.bbrc.2015.01.008 PMid:25998734.

  • 45.

    Berridge MJ. Vitamin D, reactive oxygen species and calcium signalling in ageing and disease. Philos Trans R Soc Lond B Biol Sci 2016; 371: 20150434. https://doi.org/10.1098/rstb.2015.0434 PMid:27377727 PMCid:PMC4938033.

  • 46.

    Wiseman H. Vitamin D is a membrane antioxidant Ability to inhibit irondependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Lett 1993; 326: 285-288. https://doi.org/10.1016/0014-5793(93)81809-E.

  • 47.

    Mohammed MA, Aboulhoda BE, Mahmoud RH. Vitamin D attenuates gentamicin-induced acute renal damage via prevention of oxidative stress and DNA damage. Hum Exp Toxicol 2019; 38: 321-335. https://doi.org/10.1177/0960327118812166 PMid:30458643.

  • 48.

    Xu S, Chen YH, Tan ZX, Xie DD, Zhang C, Xia MZ, et al. Vitamin D3 pretreatment alleviates renal oxidative stress in lipopolysaccharide-induced acute kidney injury. J Steroid Biochem Mol Biol 2015; 152: 133-141. https://doi.org/10.1016/j.jsbmb.2015.05.009 PMid:26013770.

  • 49.

    Almasmoum H, Refaat B, Ghaith MM, Almaimani RA, Idris S, Ahmad J, et al. Protective effect of Vitamin D3 against lead induced hepatotoxicity, oxidative stress, immunosuppressive and calcium homeostasis disorders in rat. Environ Toxicol Pharmacol 2019; 72: 103246. https://doi.org/10.1016/j.etap.2019.103246 PMid:31465891.

  • 50.

    Moore C, Murphy MM, Keast DR, Holick MF. Vitamin D intake in the United States. J Am Diet Assoc 2004; 104: 980-983. https://doi.org/10.1016/j.jada.2004.03.028 PMid:15175600.