Survey on CCR5-Δ32 mutation in healthy individuals and patients with chronic hepatitis B referred to the clinical laboratory of Iranian Blood Transfusion Organization

How to Cite:Sajjad JalaliZohreh SharifiMohammad Hossein SanatiAbolhassan Shahzadeh FazeliSurvey on CCR5-Δ32 mutation in healthy individuals and patients with chronic hepatitis B referred to the clinical laboratory of Iranian Blood Transfusion Organization.koomesh.15(3):e153878.

Abstract

Introduction:CCR5 is one of the most important chemokine receptor involved in the recruitment of speciﬁc anti-viral immune cells to the liver. CCR5-Δ32 is a functionally null allele of CCR5 gene with a 32-bp deletion. Several studies have reported that this mutation may be associated with recovery from acute hepatitis B or persistence of HBV infection. The main purpose of this study was to compare the frequency of the Δ32 mutation within the CCR5 gene in a group of chronic HBV infected patients with healthy individuals in Iran. Materials and Methods:A total of 200 blood samples including 100 healthy individuals and 100 HBsAg positive patients were randomly selected. The samples were tested for HBsAg by ELISA and HBV-DNA by PCR method. Genomic DNA was extracted by salting out method. CCR5-Δ32 mutation was genotyped by PCR with specific primers. Results:The results of this study showed that none of the subjects in the control and patient groups had a 32-bp deletion in the CCR5 gene. CCR5-Δ32 was not a prevalent mutation in control group and HBV infected patients in Iran. Conclusion:No significant difference was observed in genotypes frequency of CCR5-Δ32 between control group and HBV infected patients, therefore it seems that this mutation is not associated with persistence of HBV infection in Iranian patients

Keywords

CCR5, Chronic hepatitis B, Mutation

CCR5، جهش Δ32، هپاتیت مزمن B، افراد سالم

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