Examination of antioxidant enzymes andmatrix metalloproteinases in conditional medium of bone marrow mesenchymal stem cells and adipose-derived stem cells in vitro

authors:

avatar Leyla Mirzaian 1 , avatar Mohammadtaghi Ghorbanian 2 , * , avatar Taghi Lashkarbolouki 2 , avatar Maryam HajiGhasemKashani 2 , avatar Rada Dehghan 3

Dept. of Histology and Emberiology, School of Biology, Damghan University, Damghan, Iran
Dept. of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
School of Biology, Damghan University, Damghan, Iran
Koomesh: Vol.15, issue 3; e153885
published online: August 21, 2014
article type: Research Article

How To Cite Mirzaian L, Ghorbanian M, Lashkarbolouki T, HajiGhasemKashani M, Dehghan R. Examination of antioxidant enzymes andmatrix metalloproteinases in conditional medium of bone marrow mesenchymal stem cells and adipose-derived stem cells in vitro. koomesh. 2014;15(3):e153885. 

Abstract

 Introduction: Mesenchymal stem sells (MSCs) have an important role in cell therapy due to those high proliferation, differentiation and cell regeneration capacity. Advent signs of Replicative Cellular Senescence (R.C.S) in culture condition are one of the problems in stem cells culture pathway before treatment. R.C.S is associated with reducing the capacity of antioxidant enzymes and matrix metalloproteinase (MMPs) proteins changes. The aim of this study was to areinvestigate and compare the production capacity of antioxidant superoxiddismutase (SOD) and glutathione peroxidase (GPx) enzymes and matrix metalloproteinases (MMP-2) in conditional medium of rat bone marrow mesenchymal stem cells (BMSCs) and adipose-derived stem cells (ADSCs). Materials and Methods: BMSCs and ADSCs were respectively isolated from the tibia and femur bones and subcutaneous adipose tissues. These cells were cultured for alternative 7 passages in α-MEM supplemented with 10% fetal bovine serum. Evaluation of antioxidant and MMP activity in the conditional medium without serum was respectively assayed by enzymatic assay and zymography. Results: Two types of cells studied almost identical have most activity in the expression of SOD and GPx, respectively, in 5 and 4-6 passages. Also, demonstrated MMP activity is highest in the fourth passage. On the other hand, in these cells, membrane health reported with measurement of MDA and LDH. Conclusion: Adipose tissue has a population of stem cells with high capacity of antioxidant. Also, BMSCs and ADSCs are at highest level of antioxidant and MMP expression capacity in 4-6 passages