Evaluation of new method to induce neuritis and comparison its symptoms with current chronic constriction injury method in male rat

Ali Ghanbari; Fatemeh Shahsavari; Hossein ali Safakhah

doi:10.5812/koomesh-154055

Koomesh

Journal of Semnan University of Medical Sciences

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Evaluation of new method to induce neuritis and comparison its symptoms with current chronic constriction injury method in male rat

Author(s):

Ali Ghanbari^1,*,

Fatemeh Shahsavari²,

Hossein ali Safakhah^{3, 4}

1- Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran

2Student research Committee, Semnan University of Medical Sciences, Semnan, Iran

3Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran

4Dept. of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran

Koomesh:Vol. 23, issue 1; e154055

Published online:Dec 24, 2021

Article type:Research Article

How to Cite:Ali GhanbariFatemeh ShahsavariHossein ali SafakhahEvaluation of new method to induce neuritis and comparison its symptoms with current chronic constriction injury method in male rat.koomesh.23(1):e154055.

Abstract

Introduction: There are various animal models to investigate neuropathic pain which the most usual one is chronic constriction injury method. The aim of the present study was the evaluation of neuropathic pain following chronic spiral suturing around the sciatic nerve without ligation as a new model. Materials and Methods: Thirty Wistar male rats, weighting 180-220 gram randomly were divided into three groups as sham, chronic constriction injury (CCI), and chronic sciatic spiral suturing (CSS). CCI was performed by four loos ligation using catgut chromic suture around sciatic nerve. CSS was performed through spirally suturing of catgut chromic four times around the sciatic nerve without any ligation. In the sham group, muscle and skin were sutured after exposing the nerve. To determine mechanical allodynia and thermal hyperalgesia, Von Frey filaments and plantar test devise were used respectively every four days during three weeks. Results: CCI led to significant mechanical allodynia (P

Keywords

Neuropathic Pain

Mechanical allodynia

Thermal Hyperalgesia.

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