Effect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method

Gilda Parsamanesh; Maryam Mehri; Mostafa sheikhzadeh; Seyyed Hossein Mousavie Anijdan; Amrollah Mostafazadeh

doi:10.5812/koomesh-154056

Koomesh

Journal of Semnan University of Medical Sciences

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Effect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method

Author(s):

Gilda Parsamanesh¹,

Maryam Mehri²,

Mostafa sheikhzadeh¹,

Seyyed Hossein Mousavie Anijdan^{3, 4},

Amrollah Mostafazadeh⁵

1Laboratory of Animal Care Center, Babol University of Medical Sciences, Babol, Iran

2- Student Research Committee, Babol University of Medical Sciences, Babol, Iran

3- Depat. of Radiation Technology, School of Allied Medical Sciences, Babol University of Medical Sciences, Babol, Iran

4Radiotherapy Center, Shahid Rajayee Hospital, Babol University of Medical Sciences, Babol, Iran

5Cellular and Molecular Biology Research Center, Research Institutes of Health, Babol University of Medical Sciences, Babol, Iran

Koomesh:Vol. 23, issue 1; e154056

Published online:Dec 24, 2021

Article type:Research Article

How to Cite:Gilda ParsamaneshMaryam MehriMostafa sheikhzadehSeyyed Hossein Mousavie AnijdanAmrollah MostafazadehEffect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method.koomesh.23(1):e154056.

Abstract

Introduction: Bone marrow and immune cell transplants are a common method of treating some blood diseases around the world. However, due to the malfunction of the immune system, its use is not always effective. In this study, we evaluated the potential of culture stress in serum-free medium in regulating the immune system. Materials and Methods: Spleen immune cells were isolated from Balb/C and C57bl/6 mice using the Ficol gradient method. C57bl/6 mice splenocytes were cultured in serum starved and non-starved conditions at indicated time points. Cell viability was determined by MTT assay. Then the allogenicity of these cells was determined by mixed lymphocytic culture (in vitro model of bone marrow transplantation) with Balb/C mouse splenocytes and cell proliferation was then examined using MTT method and necessary photographs were prepared too. Results: The viability rate of starved immune cells after 72 h was 82.1% of the control group. After 24h, the allogenicity of these cells decreased significantly compared to the control cells (P

Keywords

Transplantation

Cell culture Technique

Mouse

Immunoregulation

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