Evaluation of Antibacterial Activity of Satureja Khuzestanica J. Essential Oil against Standard and Isolated Strains of Listeria monocytogenes

Khosro Issazadeh; Soheil Shokri-Fashtali; Naser Ghaemi; Alireza Massiha; Mehdi Assmar; Sheida  Akbari-Shahabi

Zahedan Journal of Research in Medical Sciences

The Official Journal of Zahedan University of Medical Sciences

Current Issue All Issues In Press Articles Accepted Manuscripts Search

Journal Information Boards and Committees Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Contact Us Support Reviewer and AE Registration Form

APC

Authors Guide Submit Manuscript

Evaluation of Antibacterial Activity of Satureja Khuzestanica J. Essential Oil against Standard and Isolated Strains of Listeria monocytogenes

Author(s):

Khosro Issazadeh¹,

Soheil Shokri-Fashtali²,

Naser Ghaemi³,

Alireza Massiha³,

Mehdi Assmar³,

Sheida Akbari-Shahabi^3,*

2Department of Mathematics, Lahijan Branch , Islamic Azad University, Lahijan, Iran

3Department of Biotechnology, Tehran University, Tehran, Iran

3Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 16, issue 10

Published online:Jun 29, 2013

Article type:Research Article

Received:Dec 12, 2012

Accepted:Feb 10, 2013

How to Cite: Khosro Issazadeh Soheil Shokri-Fashtali Naser Ghaemi Alireza Massiha Mehdi AssmarSheida Akbari-Shahabiet al.Evaluation of Antibacterial Activity of Satureja Khuzestanica J. Essential Oil against Standard and Isolated Strains of Listeria monocytogenes.Zahedan J Res Med Sci.16(10):null-null.

Abstract

Background: The purpose of this study was to determine antibacterial activity of essential oil of Satureja khuzestanica against Listeria monocytogenes (PTCC1295) and strains isolated from breast milk show that.

Materials and Methods: In this descriptive-analytic study, Essence of leave’s plant was extracted and identified its compounds and then carvacrol was isolated. Antibacterial activities were examined by agar dilution method against L. monocytogenes. Minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) were carried out by micro dilution method. Then bacterial suspension injected the BALB/c mice. Forty-eight h after seeing the listeriosis disease signs were started the treatment. Ampicillin (10 μg/disc) and trimethoprim (5 g) were used as controls

Results: The results showed that the inhibitory zone diameter standard and essential oils for strains isolated species were respectively 59 and 50 mm. This amount was determined by carvacrol, respectively, 60 and 48 mm. Inhibition zone diameter measurements for standard strains of ampicillin and trimethoprim tedious strains, respectively, 21, 40, 18 and 33 mm, respectively. The minimum inhibitory concentration of essential oils, carvacrol and ampicillin than standard strains, respectively 1.56, 1.56 and 155×10ËÂ8 μg/mL and MBC 3.125, 3.125 and 125×10-7 μg/mL was determined by the ratio of the strain 3.125, 3.125 and 0.0062 μg/mL and MBC was 6.25, 6.25 and 0.025 μg/mL.

Conclusion: This study showed that bacterial cleansing properties of essential oil of this plant have a strong and effective combination that is carvacrol.

Keywords

Essential Oils

Carvacrol

Satureja Khuzestanica

Antimicrobial Activity

Listeria Monocytogene

Fulltext

Fulltext is availale in the PDF.

References

1.
References are available in the PDF.

comments