Prevalence of AmpC and ESBL Producing E. coli and Antibacterial Effect of Allim sativum on Clinical Isolates Collected from Zahedan Hospitals

authors:

avatar Sara Shayan 1 , avatar Mohammad Bokaeian 2 , * , avatar Shahram Shahrak 2 , avatar Saeide Saeidi 3

nfectious Diseasec and Tropical Medicin Research Center Zahedan University of Medical Scienes, Zahedan, Iran
Department of Microbiology, Zahedan University of Medical Sciences, Zahedan, Iran
Department of Biology, Faculty of Science s , Islamic Azad University, Kerman, Iran
Zahedan Journal of Research in Medical Sciences: Vol.16, issue 4; 6-10
published online: May 20, 2013
article type: Research Article
received: March 03, 2013
accepted: April 24, 2013

how to cite: Shayan S, Bokaeian M , Shahrak S, Saeidi S. Prevalence of AmpC and ESBL Producing E. coli and Antibacterial Effect of Allim sativum on Clinical Isolates Collected from Zahedan Hospitals. Zahedan J Res Med Sci. 2014;16(4): 6-10. 

Abstract

 
Background: AmpC beta-lactamases are capable to hydrolyse all beta-lactam antibiotics except cefepime and carbapenems. Herbal medicines have been important sources of products for the developing countries in treating common infectious diseases and overcome the problems of resistance and side effects of the currently available antimicrobial agents.
Materialss and Methods: Total 410 non repetitive clinical E. coli strains recovered during 7 month, were screened for AmpC production by disc diffusion test using cefoxitin (30 µg) discs and confirmed by combined disc diffusion test using phenyl boronic acid and the MIC and MBC of Allim sativum (A. sativum) alcoholic extract against AmpC positive E. coli isolates were determined.
Results: A total of 107 of 410 isolates (26%) were cefoxitin resistant and 13 (3.1%) isolates harboured AmpC enzymes. A. sativum alcoholic extract were effective against AmpC producing E. coli isolates.
Conclusion: There is need for a correct and reliable phenotypic test to identify AmpC beta lactamases and to discriminate between AmpC and ESBL producers and also these bioactive plants may help alleviate the problem of drug resistance.

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