Abstract
Background: Enterotoxins of Staphylococcus aureus are the main factors which cause food poisoning.
Matrrials and Methods: Thirty five samples of Schizothorax zarudnyi were cultured by bacteriological methods and S. aureuses were identified. The isolated bacteria were evaluated by PCR for diagnosis of the gene encoding of SEA and SEB. Data were analyzed using Stata software.
Results: PCR is a rapid, sensitive, specific and inexpensive method for detecting Staphylococcal enterotoxins in food.
Conclusion: 37.2% of fishes were contaminated by S. aureus. The PCR results showed that 14.3% of S. aureus isolates possessed the SEA gene, 8.5% had the SEB gene and 5.7% possessed both genes.
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© 2014, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.