Effect  of  Saffron  aqua  Extract  on  Angiogenesis  in  Chick Chorioalantoic Membrane

Marzieh Mousavi; Javad  Baharara; Saeideh Zafar - Balanezhad; Khadijeh Shaheokh - Abadi

Zahedan Journal of Research in Medical Sciences

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Effect of Saffron aqua Extract on Angiogenesis in Chick Chorioalantoic Membrane

Author(s):

Marzieh Mousavi¹,

Javad Baharara^2,*,

Saeideh Zafar - Balanezhad³,

Khadijeh Shaheokh - Abadi⁴

155 Zahedan Journal of Research in Medical Sciences Journal homepage: www.zjrms.ir Effect of Saffron aqua Extract on Angiogenesis in Chick Chorioalantoic Membrane M arzieh Mo u savi, 1 Javad Baharara, * 1 S aeideh Zafar - Balanezhad, 1 Kh adijeh Shaheokh - Abadi 1 1 . Dep artmen t of Biology , Faculty o f Science s , Applied Biology Center , Mashhad Branch, Islamic Azad University, Mashhad , Iran Article information Abstract Article history: Received: 4 Aug 2012 Accepted: 12 Dec 2012 Available online: 5 May 201 3 ZJRMS 201 4 ; 1 6 ( 3 ): 55 - 58 Background : S tudies confirmed anticancer properties of saffron extract . Angiogenesis, formation of new blood vessels which is necessary in many physiological stages and pathological events such as tumor growth. So it would be an effective strategy to inhibit angiogenesis to treat many cancers and metastasis. In this experimental study , effects of saffron on angiogenesis in chick chorioalantoic membrane (CAM) were investigated . Materials and Methods : Fifity r oss fertilized eggs divided in 5 groups, including : control, sham exposed, experimental group 1, 2 and 3 . In second day of incubation window was opened on eggs . I n day 8 gelatin sponges contain gelatin and albumin was put on chorioalantoic membrane and was soaked with Saffron aqua extract in concentration 100, 400 and 800 μg/ml . In 12th day all cases were photographed by photo stereomicroscope . N umbers and lengths of vessels around the sponges were measured by Image J software . Data were analyzed with SPSS - 16 in significant le vel p

3Dep artmen t of Biology , Faculty o f Science s , Applied Biology Center , Mashhad Branch, Islamic Azad University, Mashhad , Mashhad , Iran

4Dep artmen t of Biology , Faculty o f Science s , Applied Biology Center , Mashhad Branch, Islamic Azad University, Mashhad, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 16, issue 3; 55-58

Published online:May 05, 2013

Article type:Research Article

Received:Apr 12, 2012

Accepted:Dec 12, 2012

How to Cite:Marzieh MousaviJavad BahararaSaeideh Zafar - BalanezhadKhadijeh Shaheokh - AbadiEffect of Saffron aqua Extract on Angiogenesis in Chick Chorioalantoic Membrane.Zahedan J Res Med Sci.16(3):55-58.

Abstract

Background: Studies confirmed anticancer properties of saffron extract. Angiogenesis, formation of new blood vessels which is necessary in many physiological stages and pathological events such as tumor growth. So it would be an effective strategy to inhibit angiogenesis to treat many cancers and metastasis. In this experimental study, effects of saffron on angiogenesis in chick chorioalantoic membrane (CAM) were investigated.

Materials and Methods: Fifity ross fertilized eggs divided in 5 groups, including: control, sham exposed, experimental group 1, 2 and 3. In second day of incubation window was opened on eggs. In day 8 gelatin sponges contain gelatin and albumin was put on chorioalantoic membrane and was soaked with Saffron aqua extract in concentration 100, 400 and 800 µg/ml. In 12th day all cases were photographed by photo stereomicroscope. Numbers and lengths of vessels around the sponges were measured by Image J software. Data were analyzed with SPSS-16 in significant level p<0.05.

Results: According to data analysis, changes had no correlation on the average length of blood vessels in the first experimental group (41.5±5.5 mm), compared with the control group, (44.5±2.4 mm). While in the second and third experimental group (40.2±2.1 mm) and (38.4±3.8 mm) these changes were significant (p=0.001). On the other hand, the average number of blood vessels in the first experimental group (22.07±5.2) in compare with the control group (27.46±4.4) shows a significant reduction (p=0.02), this decline between the second (18.80±4.4) and third (15.87±3.8) experimental groups was significant at the level of p=0.001.

Conclusion: Saffron extract has a dose dependent inhibitory effect on angiogenesis in chick chorioalantoic membrane.

Keywords

Angiogenesis

Saffron

Cancer

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