Occurrence of Ambler Class B Metallo-β-Lactamase Gene in Imipenem-Resistant Pseudomonas Aeruginosa Strains Isolated from Clinical Samples

Zeynab  Golshani; Ali Mohammad Ahadi; Ali Sharifzadeh

Zahedan Journal of Research in Medical Sciences

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Occurrence of Ambler Class B Metallo-β-Lactamase Gene in Imipenem-Resistant Pseudomonas Aeruginosa Strains Isolated from Clinical Samples

Author(s):

Zeynab Golshani^1,*,

Ali Mohammad Ahadi²,

Ali Sharifzadeh³

1Department of Microbiology, Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

2Department of Genetic, School of Science, Shahr-e-kord University, Shahr-e-K ord, Iran

3Department of Microbiology, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 16, issue 2; 6-9

Published online:May 05, 2013

Article type:Research Article

Received:Dec 07, 2012

Accepted:Feb 05, 2013

How to Cite:Zeynab GolshaniAli Mohammad AhadiAli SharifzadehOccurrence of Ambler Class B Metallo-β-Lactamase Gene in Imipenem-Resistant Pseudomonas Aeruginosa Strains Isolated from Clinical Samples.Zahedan J Res Med Sci.16(2):6-9.

Abstract

Background: Metallo-β-lactamase (MBLs) can hydrolyze a broad spectrum of beta-lactams, including penicillins, cephalosporins, and carbapenems. Genes encoding these enzymes are located on the plasmid that can easily be transferred to other bacteria. The aim of this study was to isolate and identify the Pseudomonas aeruginosa strains encoding VIM1 gene, in clinical samples, using the PCR technique.

Maerials and Methods: During a 4 month period, 100 strains of Pseudomonas aeruginosa from clinical specimens were collected. Standard tests were performed to identify strains of Pseudomonas aeruginosa. Resistance to antibiotics was examined and then the PCR was used to detect VIM1gene.

Results: In this study, the highest rates of resistance to antibiotics, amikacin and cefotaxime was observed (65% and 62%), the lowest resistance to antibiotics piperacillin (48%) and imipenem and cefepime with 55% resistance was reported. DDST method was performed for 37 strains for the MBl detection. Among the 37 isolate, 30 strains were MBL-producing with imipenem-EDTA method. Twelve strains (18%) were carriers of VIM1 gene using the PCR method.

Conclusions: In the present study, the prevalence of strains producing MBL genes in strains of hospitals is a growing trend; correct prescription of medications can prevent the spread of resistant pathogens. It is suggested that molecular methods for rapid detection of resistance genes can be used to prevent the spread of this genes.

Keywords

Beta-Lactamase

Pseudomonas Aeruginosa

Antibiotic Resistance

PCR

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