Comparison of Culture and Multiplex PCR Technique for Detection of Brucella abortus and Brucella melitensis from Human Blood Samples

Reza Mirnejad; Ali Reza Vahdati; Ali Ahmadi; Seied Mojtaba Mortazavi; Vahhab Piranfar

Zahedan Journal of Research in Medical Sciences

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Comparison of Culture and Multiplex PCR Technique for Detection of Brucella abortus and Brucella melitensis from Human Blood Samples

Author(s):

Reza Mirnejad¹,

Ali Reza Vahdati²,

Ali Ahmadi¹,

Seied Mojtaba Mortazavi³,

Vahhab Piranfar^4,*

1Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland

3Health Station, Kerman University of Medical Sciences, Kerman, Iran

4Department of Microbiology, Tonekabon Branch, Islamic Azad University of Tonekabon, Tonekabon, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 15, issue 12; e4325

Published online:Feb 12, 2013

Article type:Research Article

Received:Nov 27, 2012

Accepted:Jan 09, 2013

How to Cite:Mirnejad R, Vahdati A R, Ahmadi A, Mortazavi S M, Piranfar V. Comparison of Culture and Multiplex PCR Technique for Detection of Brucella abortus and Brucella melitensis from Human Blood Samples.Zahedan J Res Med Sci.2013;15(12):e4325.

Abstract

Background: To compare culture methods with multiplex PCR technique for identification of Brucella abortus and Brucella melitensis from suspicious patients with clinical history of brucellosis and positive serological test (Rose Bengal test and serum agglutination test).

Materials and Methods: In this study, 160 blood samples from patients suspected of Brucellosis with high serum titers of 1/80 were studied. All samples were cultured in Brucella-specific media. Brucella species were identified by using microbiological methods. DNA was extracted with Phenol-chloroform DNA extraction method. IS711 was amplified simultaneously using three specific primers and obtained patterns were analyzed.

Results: From 160 samples, 47.5% (76) were culture positive cases from which 43 cases were B. melitensis and 33 were B. abortus With the PCR technique 108 were detected positive from which 45.3% were B. abortus and 54.6% were B. melitensis. It should be noted that all 76 samples with positive culture were also identified by PCR.

Conclusion: Generally, use of the molecular technique multiplex PCR in addition to increased speed and accuracy and less false results than bacterial culture method, is able to identify different species of brucella. This will facilitate the treatment process.

Keywords

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