Prevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using Cultural and PCR Methods

Reza Shahrokhabadi; Ebrahim Rahimi; Hassan Mommtaz; Rahele Poursahebi

Zahedan Journal of Research in Medical Sciences

The Official Journal of Zahedan University of Medical Sciences

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Prevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using Cultural and PCR Methods

Author(s):

Reza Shahrokhabadi^1,*,

Ebrahim Rahimi²,

Hassan Mommtaz³,

Rahele Poursahebi⁴

1Young Researchers Club, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran

2Department of Food Hygiene, Faculty of Veterinary Medicine, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran

3Department of Microbiology, Faculty of Veterinary Medicine, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran

4Department of Food Hygiene, Faculty of Veterinary Medicine, Bahonar University, Kerman, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 15, issue 12; e4331

Published online:Feb 25, 2013

Article type:Brief Report

Received:Nov 15, 2012

Accepted:Jan 30, 2013

How to Cite:Reza ShahrokhabadiEbrahim RahimiHassan MommtazRahele PoursahebiPrevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using Cultural and PCR Methods.Zahedan J Res Med Sci.15(12):e4331.

Abstract

Background: Campylobacter species are common bacterial pathogens causing gastroenteritis in humans worldwide.

Materials and Methods: A total of 148 randomly sheep carcasses were sampled by surface section of neck meat taken immediately after slaughter analyzed using microbiological examinations.

Results: Campylobacter spp. was isolated from 10.13% meat cultures samples examined. Among these 80% sample were C. jejuni and 20% sample were C. coli. Using PCR assays, the number of positive campylobacters increased to 11.48%. Of these positive samples, 82.35% were C. jejuni and 17.65% were C. coli. Significantly higher prevalence rates of Campylobacter spp. (p<0.05) were found in the meat samples taken in summer (47.05%).

Conclusion: The PCR is a reliable and sensitive method which can be used as a diagnostic technique for the detection of campylobacter in lamb samples.

Keywords

Campylobacter jejuni Campylobacter coli

PCR

Sheep

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