Abstract
Background:
In acute myeloid leukemia (AML), a large number of tumor suppressor genes are silenced through DNA methylation such as CDKN2B and p73. Wnt inhibitory factor 1 (WIF1) and Dickkopf-1 (DKK-1) are negative regulator of the Wnt signaling pathway.Objectives:
In the present study, we studied the methylation status of WIF1 and DKK-1 genes in AML patients.Patients and Methods:
In this case-control study, blood samples from 120 AML patients and 25 healthy control subjects collected, isolated DNA was treated with sodium bisulphite and examined by methylation specific PCR (MS-PCR) with primers specific for methylated and unmethylated sequences of the WIF1 and DKK-1 genes.Results:
The frequency of aberrant hypermethylation of WIF1 and DKK-1 genes in AML patients determined 35% (42/120) and 28.3% (34/120), respectively. In addition, for all subjects in control group, methylation of WIF1 and DKK-1 genes were negative. Patients with M0 subtype of French-American-British (FAB)-AML had the highest incidence of hypermethylation of WIF1 (P = 0.003) and DKK-1 (P = 0.005) genes.Conclusions:
The present study showed that, like many solid tumors, WIF1 and DKK-1 genes methylation also occurs in AML. The study of other antagonists of Wnt signaling pathway are recommended.Keywords
Acute Myeloid Leukemia Wnt Inhibitory Factor 1 Dickkopf-1 DNA Methylation
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Copyright
Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.