Effect of Cytosolic Extract of Alternaria Alaternata Fungus on Monocyte-Derived Dendritic Cell Phagocytosis Ability and T-Lymphocyte Proliferation in the Presence of Myelin Basic Protein

Alireza  Loghmani; Nowruz Delirezh; Abdolghaffa Ownagh; Hadi Mohebalian

Zahedan Journal of Research in Medical Sciences

The Official Journal of Zahedan University of Medical Sciences

Current Issue All Issues In Press Articles Accepted Manuscripts Search

Journal Information Boards and Committees Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Contact Us Support Reviewer and AE Registration Form

APC

Authors Guide Submit Manuscript

Effect of Cytosolic Extract of Alternaria Alaternata Fungus on Monocyte-Derived Dendritic Cell Phagocytosis Ability and T-Lymphocyte Proliferation in the Presence of Myelin Basic Protein

Author(s):

Alireza Loghmani^1,*,

Nowruz Delirezh¹,

Abdolghaffa Ownagh¹,

Hadi Mohebalian¹

1Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 15, issue 11; e92793

Published online:Jan 15, 2013

Article type:Research Article

Received:Jul 03, 2012

Accepted:Oct 20, 2012

How to Cite:Alireza LoghmaniNowruz DelirezhAbdolghaffa OwnaghHadi MohebalianEffect of Cytosolic Extract of Alternaria Alaternata Fungus on Monocyte-Derived Dendritic Cell Phagocytosis Ability and T-Lymphocyte Proliferation in the Presence of Myelin Basic Protein.Zahedan J Res Med Sci.15(11):e92793.

Abstract

Background: Multiple sclerosis (MS) is an autoimmune disease with impairment in function of CNS, meanwhile macrophages and dendritic cells (DC) can cause inflammation and damage to the myelin of nerve cells by releasing Reactive oxygen species (ROS) and other harmful substances when these cells get matured. We investigated the effect of Alternaria alternata (A. alternata) extract on phagocytic T cell stimulation activity of DC pulsed with Myelin Basic Protein (MBP) as a laboratory model of MS.
Materials and Methods: Plastic adherent monocytes were cultured with granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 for converting these cells to MoDc (Monocyte-Derived Dendritic Cell), pulsed with MBP, matured in the presence of monocyte-conditioned medium (MCM) in control group and MCM+Alternaria. alternata extract in treatment groups. Phagocytic activity of DC was evaluated and T cell responses were investigated by MTT test.
Results: Phagocytic activity in treatment groups decreased significantly in compare with control group. Meanwhile, DC couldn’t stimulate T cell proliferation.
Conclusion: A. alternata extract decreased phagocytic activity of MoDc-pulsed with MBP and had no effect on T cell proliferation may provide a new strategy on immunotraphy of multiple sclerosis.

Keywords

Alternaria alternate

Extract fungus

Myelin Basic Protein (MBP)

Dendritic cell

Maturation

Lymphocyte T

Fulltext

The full text of this article is available on the PDF file.

References

1.
The References of this article are available on the PDF file.

comments