Identifying Human Papilloma Virus (HPV) in Women with Genital Warts by Multiplex–PCR Method

Haniyeh Bashizadeh-Fakhar; Masood  Ghane; Roya Faraji; Behrang Ashoorizadeh

Zahedan Journal of Research in Medical Sciences

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Identifying Human Papilloma Virus (HPV) in Women with Genital Warts by Multiplex–PCR Method

Author(s):

Haniyeh Bashizadeh-Fakhar¹,

Masood Ghane^1,*,

Roya Faraji²,

Behrang Ashoorizadeh³

1Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

2Department of Gynecology, Guilan University of Medical Sciences, Guilan, Iran

3Department of Surgery, Guilan University of Medical Sciences, Guilan, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 15, issue 10; e92832

Published online:May 11, 2013

Article type:Research Article

Received:Nov 15, 2012

Accepted:Feb 12, 2013

How to Cite:Haniyeh Bashizadeh-FakharMasood GhaneRoya FarajiBehrang AshoorizadehIdentifying Human Papilloma Virus (HPV) in Women with Genital Warts by Multiplex–PCR Method.Zahedan J Res Med Sci.15(10):e92832.

Abstract

Background : Many studies have shown that some types of Human Papillomavirus (HPV) play an important role in cervix cancer which has been identified in 99% of cervix cancers worldwide. This study aimed at identifying the frequency of high-risk types of this virus in cervical lesions among patients referred to Al-Zahra Hospital, Rasht.
Materials and Methods : Forty five vaginal and cervical swap samples of women with genital warts were tested considering the presence of DNA of 14 common HPV types by using 3 series of specific PCR.
Results : Out of 45 vaginal swap samples of women suffering genital warts, 37 samples (82.2%) were reported with 95% CI (70.6-93.83) considering the presence of HPV. The frequency distribution of HPV types in 45 patients with genital wart were as follows: 5 cases (13.5%) with HPV35, 4 cases (10.8 %) with HPV16, 4 cases (10.8 %) with HPV31, 3 cases (8.1 %) with HPV33, 3 cases (8.1 %) with HPV52, 3 cases (8.1 %) with HPV58, 2 cases (5.4 %) with HPV45, 1 case (2.7 %) with HPV18, 1 case (2.7 %) with HPV59, and 1 case (2.7 %) with HPV39.
Conclusion : Inasmuch as samples of our study have been collected from one of the most important women-referring medical centers (Al-Zahra Medical and Teaching Center) in Guilan province, thus the results can be used for screening, management, and vaccination of the target population against the common types of virus in Guilan.

Keywords

Human Papillomavirus (HPV)

stereotypes

genital warts

multiplex PCR

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