Identifying Human Papilloma Virus (HPV) in Women with Genital Warts by Multiplex–PCR Method

authors:

avatar Haniyeh Bashizadeh-Fakhar 1 , avatar Masood Ghane 1 , * , avatar Roya Faraji 2 , avatar Behrang Ashoorizadeh 3

Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Department of Gynecology, Guilan University of Medical Sciences, Guilan, Iran
Department of Surgery, Guilan University of Medical Sciences, Guilan, Iran
Zahedan Journal of Research in Medical Sciences: Vol.15, issue 10; e92832
published online: May 11, 2013
article type: Research Article
received: November 15, 2012
revised: January 08, 2013
accepted: February 12, 2013

how to cite: Bashizadeh-Fakhar H, Ghane M , Faraji R, Ashoorizadeh B. Identifying Human Papilloma Virus (HPV) in Women with Genital Warts by Multiplex–PCR Method. Zahedan J Res Med Sci. 2013;15(10):e92832. 

Abstract

Background : Many studies have shown that some types of Human Papillomavirus (HPV) play an important role in cervix cancer which has been identified in 99% of cervix cancers worldwide. This study aimed at identifying the frequency of high-risk types of this virus in cervical lesions among patients referred to Al-Zahra Hospital, Rasht.
Materials and Methods : Forty five vaginal and cervical swap samples of women with genital warts were tested considering the presence of DNA of 14 common HPV types by using 3 series of specific PCR.
Results : Out of 45 vaginal swap samples of women suffering genital warts, 37 samples (82.2%) were reported with 95% CI (70.6-93.83) considering the presence of HPV. The frequency distribution of HPV types in 45 patients with genital wart were as follows: 5 cases (13.5%) with HPV35, 4 cases (10.8 %) with HPV16, 4 cases (10.8 %) with HPV31, 3 cases (8.1 %) with HPV33, 3 cases (8.1 %) with HPV52, 3 cases (8.1 %) with HPV58, 2 cases (5.4 %) with HPV45, 1 case (2.7 %) with HPV18, 1 case (2.7 %) with HPV59, and 1 case (2.7 %) with HPV39.
Conclusion : Inasmuch as samples of our study have been collected from one of the most important women-referring medical centers (Al-Zahra Medical and Teaching Center) in Guilan province, thus the results can be used for screening, management, and vaccination of the target population against the common types of virus in Guilan.

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References

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