The Effect of Reduced Bacterial Dilution on Human Amniotic Membrane Antibacterial Activity, in Vitro

Mohammad Mehdi Soltan Dallal; Zohre Kalafi; Abdolaziz Rastegar-Lari; S.Kazem Hosseini; Abbas Rahimi-Foroushani; Zahra Deilami-Khiabani; Siamak Heidarzadeh

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The Effect of Reduced Bacterial Dilution on Human Amniotic Membrane Antibacterial Activity, in Vitro

Author(s):

Mohammad Mehdi Soltan Dallal^1,*,

Zohre Kalafi²,

Abdolaziz Rastegar-Lari³,

S.Kazem Hosseini⁴,

Abbas Rahimi-Foroushani⁵,

Zahra Deilami-Khiabani⁶,

Siamak Heidarzadeh¹

1Department of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran

2Department of Microbiology, Islamic Azad University, Zanjan Branch, Zanjan, Iran

3Antimicrobial Resistant Research Center, Tehran University of Medical Sciences, Tehran, Iran

4Transplant Bank, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran

5Department of Epidemiology and Biostatistics, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran

6Biological Research Center, Islamic Azad University, Zanjan Branch, Zanjan, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 15, issue 5; e92968

Published online:Nov 18, 2012

Article type:Research Article

Received:Sep 11, 2011

Accepted:Apr 26, 2012

How to Cite:Mohammad Mehdi Soltan DallalZohre KalafiAbdolaziz Rastegar-LariS.Kazem HosseiniAbbas Rahimi-ForoushaniZahra Deilami-KhiabaniSiamak Heidarzadehet al.The Effect of Reduced Bacterial Dilution on Human Amniotic Membrane Antibacterial Activity, in Vitro.Zahedan J Res Med Sci.15(5):e92968.

Abstract

Background : The human amniotic membrane is the inner most layer of placenta and has antimicrobial effect, due to the presence of human beta-defensins and elafins. The purpose of this study is to investigate the effect of dilution reduction of 0.5 McFarland prepared from standard bacterial strains of Salmonella enterica BAA-708, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Klebsiella pneumoniae ATCC7881, and Enterococcus faecalis ATCC29212 on antibacterial effect of human amniotic membranes in vitro.
Materials and Methods : The amniotic membranes were obtained from the bank of organ transplantation in Imam Khomeini hospital, of women with elective cesarean section whose HIV, HBV, HCV and VDRL serological tests were negative. They were cut to 1.5×1.5 cm pieces. Then 0.5 McFarland suspensions of 1.5×108, 0.5×107 and 1.5×106 dilutions were prepared from bacteria which then were spread on Mueller Hinton medium agar and a piece of membrane was put in the center of each plate. After 24 hours incubation at 37ᵒC, the results were observed.
Results : In 0.5 McFarland standard dilution an inhibition zone was created in three standard strains of Pseudomonas aeruginosa, Escherichia coli, and Salmonella enterica unlike the other two strains. There was no change in the above results with two other dilutions and inhibition zone of sensitive strains was not created.
Conclusion : Dilution reduction of microbial strains does not affect the antibacterial impact of amniotic membrane and dilution reduction does not yield to a false positive response and the conversion of resistant to sensitive strains.

Keywords

Amniotic membrane

Antibacterial activity

In-vitro

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