Histological Study of Interaction between Blastema Tissue and Decellularized Three -Dimensional Matrix of Bladder

authors:

avatar Javad Baharara 1 , * , avatar Naser Mahdavishahri 2 , avatar Negar Saghiri 3 , avatar Hashem Rasti 3

Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad,Iran
Department of cell & developmental biology, Mashhad Branch, Islamic Azad University, Young Researchers Club, Mashhad, Iran

how to cite: Baharara J , Mahdavishahri N, Saghiri N, Rasti H. Histological Study of Interaction between Blastema Tissue and Decellularized Three -Dimensional Matrix of Bladder. Zahedan J Res Med Sci. 2012;14(7):e93303.

Abstract

Background: The purpose of this study is to determine the interaction between Blastema tissue and decellularized three-dimensional matrix of ovine bladder in vitro.
Materials and Methods: In order to produce relaxation of decellularized ovine bladder, combination of physiochemical methods were used. Thus, pieces of bladder were put in -4 C and then samples were put in liquid nitrogen then all samples were decellularized using chemical combination of 1% SDS (Sodium Dodecyl Sulfate). To produce Blastema tissue, a few 2 mm diameter holes were made in auricles of male New Zealand rabbits. After 72 hours, The Blastema ring was removed and it was put under sterile conditions of decellularized scaffolds inside the Blastema ring and moved to the medium. After histological stages, medium samples were stained with Hematoxylin-Eosin and Peak Indigo Carmine. The quantitative data were analyzed by ANOVA and TUKY test at the level of p<0.05.
Results: Light microscopic studies at different culture days showed that blastema cells migrated into the decellularized matrix of bladder so that the maximum migration to the scaffold occurred on the 20th day (p=0.001). Blastema cells differentiation into the epithelial, fibroblasts and adipocyte cells is obvious on the day 15 and 20.
Conclusion: Bladder decellularized matrix can be a suitable scaffold to induce and differentiate blastema cells.

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