Comparing of Cu/Zn SOD Gene Expression of Lymphocyte Cell and Malondialdehyde Level in Active Men and Women after Physical Training

Bakhtiar Tartibian; Behrouz  Baghaiee; Behzad  Baradaran; Mohammadreza  Aliparasty; Shohreh  Almasy

Zahedan Journal of Research in Medical Sciences

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Comparing of Cu/Zn SOD Gene Expression of Lymphocyte Cell and Malondialdehyde Level in Active Men and Women after Physical Training

Author(s):

Bakhtiar Tartibian^1,*,

Behrouz Baghaiee¹,

Behzad Baradaran²,

Mohammadreza Aliparasty³,

Shohreh Almasy³

1Department of Exercise Physiology, School of Physical Education, Department of exercise physiology, Urmia University, Urmia, Iran

2Department of Immunology, Tabriz University of Medical Sciences, Immunology research center, Tabriz, Iran

3Department of Immunology, Tabriz University of Medical Sciences, Department of immunology, Tabriz, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 14, issue 7; e93311

Published online:Nov 10, 2011

Article type:Research Article

Received:Aug 06, 2011

Accepted:Sep 03, 2011

How to Cite:Bakhtiar TartibianBehrouz BaghaieeBehzad BaradaranMohammadreza AliparastyShohreh AlmasyComparing of Cu/Zn SOD Gene Expression of Lymphocyte Cell and Malondialdehyde Level in Active Men and Women after Physical Training.Zahedan J Res Med Sci.14(7):e93311.

Abstract

Background: The purpose of this study is to compare Cu/Zn SOD mRNA and MDA level as a result of a session incremental exercise in active women and men.
Materials and Methods : This research is a quasi-experimental study with repeated measurements in which 14 active female and 13 male subjects with age range 22-24 participated voluntarily. Then, blood was taken from brachial vein of the subjects in three stages before and after GXT (Graded exercise test) and 3 hours after that and SYBER Green PCR Master mix reagent Kit and Real time-PCR were used to measure Cu/Zn SOD mRNA and spectrophotometer was used to measure MDA level.
Results: MDA levels increased significantly in men during the recovery stage and after the exercise (p1=0.012 and p2 =0.014), but it did not increase significantly in active women. Also, MDA difference between the two genders was not reported significant in any of the exercise stages. Cu/Zn SOD gene expression did not increase significantly in either sex.
Conclusion: The risk of injury from free radicals is more probable in active men than active women and vigorous physical activity does not significantly increase the Cu/Zn SOD gene expression.

Keywords

Gene expression

Super oxidase dismutase

Oxidative stress

Active people

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