The Inhibitory Effect of Camellia sinensis Extract on Decreasing Inductive Teratogenicity of Low Frequency Electromagnetic Field in Liver and Spleen of Balb/C Rat Embryo

authors:

avatar Javad Baharara 1 , * , avatar Naser Mahdavishahri 2 , avatar Saeedeh Zafar-Balanejad 3 , avatar Esmat Kamareh 4

Department of Animal Developmental Biology, Islamic Azad University, Mashhad branch, Mashhad, Iran
Department of Biology, Islamic Azad University, Mashhad branch, Mashhad, Iran
Department of Biology, School of Sciences, Islamic Azad University, Mashhad branch, Mashhad, Iran
Department of Cell & Developmental Biology, Islamic Azad University, Mashhad Branch, Mashhad, Iran
Zahedan Journal of Research in Medical Sciences: Vol.14, issue 5; e93439
published online: January 07, 2012
article type: Research Article
received: July 28, 2011
accepted: October 29, 2011

How To Cite Baharara J , Mahdavishahri N, Zafar-Balanejad S, Kamareh E. The Inhibitory Effect of Camellia sinensis Extract on Decreasing Inductive Teratogenicity of Low Frequency Electromagnetic Field in Liver and Spleen of Balb/C Rat Embryo. Zahedan J Res Med Sci. 2012;14(5):e93439. 

Abstract

Background: Many studies have an emphasis on Reactive Oxygen Species (ROS) formation by electromagnetic field. Camellia sinensis is enriched with antioxidants and the antioxidants can neutralize the effects of ROS. In this study, the effect of Camellia sinensis extract on decreasing the inductive teratogenicity of the electromagnetic field (frequency 50Hz and intensity 50G) in liver and spleen of Balb/C embryonic rat is examined.
Materials and Methods: Twenty-four heads of pregnant female rat (Balb/C) were divided into four groups: control group, experimental test group (off-device), empirical group1 (electromagnetic field 50 Gauss), empirical group2 (treated using Camellia sinensis extract + electromagnetic field 50 Gauss), in this empirical-experimental study. Then, liver and spleen tissue cross sections of 19-day embryos were prepared for histological assessments after weight and Crown-Rump length were measured. Resulting quantitative data was analyzed using ANOVA statistical tests and Tukey test with the significance level (p<0.05).
Results: In examination of tissue sections, mean lymphocyte number of spleen in empirical group 1 showed a significant difference (p=0.001) comparing to the experimental control group, whereas mean lymphocyte number of spleen in empirical group 1 showed a significant difference (p=0.001) comparing to empirical group 2. Mean number of liver hepatocytes empirical samples 2 showed a significant decrease (p=0.004) comparing to the experimental control group, and mean number of liver hepatocytes empirical samples 2 showed a significant decrease (p=0.042) comparing to empirical samples 1.
Conclusion: Consumption of Camellia sinensis could compensate for the inductive impairments in many cells electromagnetic fields, but it is not recommended in pregnancy period cause of changing the number of some cells.

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