Abstract
Background: At present, nanosilver particles are used against a broad spectrum of microbial agents. The objective of this study was to evaluate the anti-leishmanial effect of nanosilver solutions on L. tropica promastigotes as compared with the trivalent antimony compound by colorimetric assay.
Materials and Method: Promastigots of L. tropica were cultured in RPMI-1640 consisting of 10% fetal calf serum (FCS) and antibiotics at 24±10C. The effect of various concentrations of nanosilver solutions was evaluated in comparison with tartar emetic. Optical density as the result of tetrazolium salts (MTT) reduction by the promastigotes into a formozan product and it was calculated by Elisa reader and IC50 (50% inhibitory concentration), subsequently.
Results: Both, nanosilver particles and tartar emetic inhibited the growth of L. tropica promastigotes. The IC50 for nanosilver solutions was significantly higher (14.9 µg/mL) than the corresponding value (5.3 µg/mL) for the control drug (p<0.001).
Conclusion: Since nanosilver particles showed an effective anti-leishmanial activity, further works are required to evaluate the effect in animal model or human volunteers.
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Copyright
© 2011, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.