Anti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay

Ahmad Khosravi; Iraj Sharifi; Mohammad Barati; Mahdi Zarean; Marayam Hakimi-Parizi

Zahedan Journal of Research in Medical Sciences

The Official Journal of Zahedan University of Medical Sciences

Current Issue All Issues In Press Articles Accepted Manuscripts Search

Journal Information Boards and Committees Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Contact Us Support Reviewer and AE Registration Form

APC

Authors Guide Submit Manuscript

Anti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay

Author(s):

Ahmad Khosravi¹,

Iraj Sharifi^2,*,

Mohammad Barati³,

Mahdi Zarean⁴,

Marayam Hakimi-Parizi⁵

1MPH student &DVM, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

2Proffesor of Parasitology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

3PhD student of Parasitology, School of Hygiene, Tehran University of Medical Sciences, Tehran, Iran.

4Instructor of Parasitology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

5Instructor of Medical Entomology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Zahedan Journal of Research in Medical Sciences:Vol. 13, issue 7; e93813

Published online:May 19, 2011

Article type:Research Article

Received:Jul 04, 2011

Accepted:Apr 10, 2011

How to Cite:Ahmad KhosraviIraj SharifiMohammad BaratiMahdi ZareanMarayam Hakimi-PariziAnti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay.Zahedan J Res Med Sci.13(7):e93813.

Abstract

Background: At present, nanosilver particles are used against a broad spectrum of microbial agents. The objective of this study was to evaluate the anti-leishmanial effect of nanosilver solutions on L. tropica promastigotes as compared with the trivalent antimony compound by colorimetric assay.

Materials and Method: Promastigots of L. tropica were cultured in RPMI-1640 consisting of 10% fetal calf serum (FCS) and antibiotics at 24±1⁰C. The effect of various concentrations of nanosilver solutions was evaluated in comparison with tartar emetic. Optical density as the result of tetrazolium salts (MTT) reduction by the promastigotes into a formozan product and it was calculated by Elisa reader and IC₅₀ (50% inhibitory concentration), subsequently.

Results: Both, nanosilver particles and tartar emetic inhibited the growth of L. tropica promastigotes. The IC₅₀ for nanosilver solutions was significantly higher (14.9 µg/mL) than the corresponding value (5.3 µg/mL) for the control drug (p<0.001).

Conclusion: Since nanosilver particles showed an effective anti-leishmanial activity, further works are required to evaluate the effect in animal model or human volunteers.

Keywords

Leishmania tropica

nanosilver particles

colorimetric assay

Fulltext

The full text of this article is available on the PDF file.

References

1.
The References of this article are available on the PDF file.

comments