Frequency of β-lactamase enzyme and antibiogram pattern in bacterial flora isolated from staffs hands

shilla jalaloor; Rooha Kasra-Kermanshahi; Ashraf-Sadat Nouhi; Hamid Zarkesh-Esfahani

Zahedan Journal of Research in Medical Sciences

The Official Journal of Zahedan University of Medical Sciences

Current Issue All Issues In Press Articles Accepted Manuscripts Search

Journal Information Boards and Committees Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Contact Us Support Reviewer and AE Registration Form

APC

Authors Guide Submit Manuscript

Frequency of β-lactamase enzyme and antibiogram pattern in bacterial flora isolated from staffs hands

Author(s):

shilla jalaloor^1,*,

Rooha Kasra-Kermanshahi²,

Ashraf-Sadat Nouhi³,

Hamid Zarkesh-Esfahani⁴

1Instructor of Microbiology, Islamic Azad University, Shahreza Branch, Young Researchers Club, Isfahan, Iran.

2Professor of Biology, School of Basic Sciences, University of Alzahra, Tehran, Iran.

3Professor of Biology, School of Sciences, Tehran University, Tehran, Iran.

4Associate Professor of Biology, School of Sciences, Isfahan University, Isfahan, Iran.

Zahedan Journal of Research in Medical Sciences:Vol. 13, issue 7; e93824

Published online:May 21, 2011

Article type:Brief Report

Received:May 25, 2010

Accepted:Jul 13, 2010

How to Cite:shilla jalaloorRooha Kasra-KermanshahiAshraf-Sadat NouhiHamid Zarkesh-EsfahaniFrequency of β-lactamase enzyme and antibiogram pattern in bacterial flora isolated from staffs hands.Zahedan J Res Med Sci.13(7):e93824.

Abstract

Background: β-lactamase is an enzyme that can inactivate β–Lactam family antibiotics. High prevalence of β-lactamase producer bacteria on the staff hands, due to antibiotic resistance and nosocomial infection in hospitalized patients. The objective of this study was to assess the frequency of β-lactamase positive bacteria and antibiogram pattern in bacterial flora isolated from staff hands of the Al-Zahra hospital in Isfahan.

Materials and Method: This laboratory research was performed during of 2005-2007 in Al-Zahra hospital in Isfahan. According to statistical formula, we randomly selected 80 samples from staff hands. Staff hand samples collected with finger print method. Bacterial identification was performed with microbiological methods and β–lactamase production was performed with Acidometric method and antibiogram pattern was performed with Kirby Bauer method.

Results: According to the acidometric test results of 80 isolated staff hands, 61.85% of strains produce β–lactamase. Staphylococcus spp., Bacillus spp. and Enterobacteriaceae were the most important producers respectively (70.83%, 64.72% and 50%). According to antibiogram test results, penicillin and vancomycin had the highest and lowest resistance.

Conclusion: High frequency of β–lactamase in bacterial survey represents colonization of bacteria in staff hands may be due to facility transmission β–lactamase plasmid genes in bacteria. We suggest better hand washing in hospitals and prescription of β–lactame antibiotics was based only on antibiogram results.

Keywords

β-lactamases

drug resistance

nosocomial infection

Fulltext

The full text of this article is available on the PDF file.

References

1.
The References of this article are available on the PDF file.

comments