Species identification of the Anopheles fluviatilis complex using PCR-sequencing and phylogenetic analysis in Jiroft district, Kerman province

1MSc of Medical Entomology, Research Center for Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences and Health Services, Zahedan, Iran.

2Professor of Medical Entomology and Vector Control Group, School of Public Health, Tehran University of Medical Sciences and Health Services, Tehran, Iran.

3Instructor of Medical Entomology and Vector Control Group, School of Public Health, Tehran University of Medical Sciences and Health Services, Tehran, Iran.

Zahedan Journal of Research in Medical Sciences:Vol. 13, issue 1; e94077

Published online:Nov 30, 2010

Article type:Research Article

Received:Mar 22, 2010

Accepted:Jun 14, 2010

How to Cite:Ahmad MehravaranHasan VatandoostMohammad R. AbaiEzatodin JavadianHamideh EdalatAbdollah GrouhiFatemeh MohtaramiMohammad Ali Oshaghiet al.Species identification of the Anopheles fluviatilis complex using PCR-sequencing and phylogenetic analysis in Jiroft district, Kerman province.Zahedan J Res Med Sci.13(1):e94077.

Abstract

Background: Malaria is endemic in southeastern Iran including Jiroft district and in favorite epidemiological condition could threaten the people health. Anopheles fluviatilis complex is a malaria vector in Iran and consists of three species of S, T, and U and 7 genotypes of S, U, T₁, T₂, Y, X and V which are different invectorial capacity. The aim of this study was to identify species composition of An.fluviatilis complex using D₃ and ITS₂ sequences in Jiroft district.

Materials and Method : Mosquitoes of An. fluviatilis have been captured using total catch, pit shelter, and human/animal night baits. PCR amplification was done against the 28S D₃ and ITS₂-rDNA and the products were sequenced . The specimens were identified to species and genotype level using phylogenetic trees and comparison of sequences with entries available in GenBank.

Results: Both ITS₂ and D₃ loci were successfully amplified and produced 514 bp and 376 bp bands respectively. Sequence analysis of ITS₂ and D₃ regions revealed that the specimens of Jiroft district were matching to the Y and T₂ haplotypes of species T of the complex.

Conclusion: Anopheles fluviatilis T is the only species in the region and based on the previous studies can be incrim inated as the main malaria vector in the region.

Keywords

Plasmodium infections

Anopheles fluviatilis

PCR

rDNA

vector

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