Histopathological Study of the Eeffect of Rofecoxib on Healing of Stomach Surgical Wounds in the Rats

authors:

avatar Daryoush Mohajeri 1 , * , avatar Ghafour Mousavi 2 , avatar Ali Rezaei 3 , avatar Hossein Jodeiri 2

Associated Professor of Pathology, Dept. of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
Assistant Professor of Veterinary Surgery, Dept. of Clinical Science, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch,Tabriz, Iran.
Associated Professor of Veterinary Surgery, Dept. of Clinical Science, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
Zahedan Journal of Research in Medical Sciences: Vol.12, issue 1; e94348
published online: January 19, 2010
article type: Research Article
received: October 18, 2009
accepted: December 12, 2009

how to cite: Mohajeri D, Mousavi G, Rezaei A, Jodeiri H. Histopathological Study of the Eeffect of Rofecoxib on Healing of Stomach Surgical Wounds in the Rats. Zahedan J Res Med Sci. 2010;12(1):e94348. 

Abstract

Background: Nowadays faster healing of surgical wounds is more challenging to researchers. The aim of the present study was to histopathological evaluate of the effect of rofecoxib, a non-steroidal anti-inflammatory drug, on healing of surgical wound in rats stomach. Materials and Methods: Sixty male Wistar rats were randomly assigned into three groups (control, positive control and experimental) of twenty animals each. A gastrotomy incision with the length of 1 cm was made in greater curvature of stomach in the rats, thereafter, sutured in 2 layers. Experimental group received rofecoxib (15 mg/kg) dissolved in Dimethyl Sulfoxide (DMSO) 5% (10 mL/Kg) daily for 15 days through the gavage. Positive control and control groups received DMSO 5% (10 mL/Kg) and normal saline (10 mL/Kg), respectively, in the same manner. Histopathological studies and comparison of wound healing among the groups were carried out considering more effective agents on wound healing including proliferation of fibroblasts, angiogenesis, re-epithelialization and collagen organization in healing tissue. Significant differences among the groups were determined by one-way analysis of variance followed by Bonferroni post-test. Statistical significance was considered at p<0.05.
Results: The numbers of fibroblasts, capillary buds and organized collagen content in healing site of experimental group were significantly more than positive control and control group (p<0.001). The size of epithelial gap in experimental group was significantly lesser than two other groups (p<0.001). There were not any significant statistical differences between the control and positive control groups from the viewpoint of the above parameters.
 Conclusion: The results showed that rofecoxib possesses a beneficial effect on healing of stomach surgical wounds in rats

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