Comparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita

Mahnaz Kesmati; Hamid Galehdari; Azar Massah

Zahedan Journal of Research in Medical Sciences

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Comparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita

Author(s):

Mahnaz Kesmati^1,*,

Hamid Galehdari²,

Azar Massah³

1Associate Prof, Dept of Biology, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

2Associate Prof, Dept of Genetic, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

3Master of Science, Dept of Biology, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 11, issue 2; e94397

Published online:Jul 15, 2009

Article type:Research Article

Received:Jun 02, 2008

Accepted:Mar 14, 2009

How to Cite:Mahnaz KesmatiHamid GalehdariAzar MassahComparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita.Zahedan J Res Med Sci.11(2):e94397.

Abstract

Background: There are some evidences that indicate there are sexual differences in drug abuse
and response to synthetic and herbal drugs. It has been shown that the expression of C-FOS
increases in many areas of brain during morphine withdrawal. Concerning the sedative effect of
Matricaria recutita extract, the aim of this study was to compare expression of C-FOS transcription
factor during morphine withdrawal with and without acute administration of Matricaria recutita on
male and female adult mice.
Materials and Methods: This study was done at Shahid Chamran University of Ahvaz in 2007 on
NMRI mice. Male and female mice were assigned into 8 groups (morphine + saline; morphine +
naloxone; morphine + Matricaria recutita + naloxone; and morphine + saline + naloxone). To
develop morphine dependency, increasing doses of morphine (20, 40, 80 mg/kg injected
subcutaneously) for 4 days. Mice received a final morphine injection (40 mg/kg) 3hours prior to
naloxone (5 mg/kg) on the day of testing (day 4). Matricaria recutita extract whit a dose of 30
mg/kg was administered intraperitoneally 5 minutes before naloxone injection. In cellular study,
90minute after naloxone injection, mice were decapitated and their brains were separated, then
mRNA was extracted from brain tissue. Using DIG-labeled DNA probe of C-FOS, beta-actin and
dot blot technique, expression of C-FOS was analyzed by Zero Dscan software. Statistical
evaluation of data was performed using student t-test and ANOVA with one factor followed by
Duncan test in SPSS software. P values less than 0.05 were considered significant.
Results: The rate of expression of C-FOS increased in male mice but decreased significantly in
female mice after naloxone-precipitated abstinence) P<0.01) . Matricaria recutita attenuated the
rate of expression of C-FOS in male mice but it showed synergistic effect on it in female mice
( P<0.05) .
Conclusion: It seems that the cellular processes involving morphine dependency and response to
treatment with Matricaria recutita are sex dependent and are affected by sexual hormones.

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